Fast responsive crystalline colloidal array photonic crystal glucose sensors
Fast responsive crystalline colloidal array photonic crystal glucose sensors
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DOI：
10.1021/ac060643i
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发表时间：
2006-07-15
影响因子：
7.4
通讯作者：
Asher, Sanford A.
中科院分区：
文献类型：
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作者：
Ben-Moshe, Matti;Alexeev, Vladimir L.;Asher, Sanford A.
我们开发了新的光子晶体聚合晶体胶体阵列（PCCA）葡萄糖传感材料，其工作的基础上形成的交联的水凝胶。这些材料由水凝胶组成，水凝胶嵌入类似于100 nm直径的单分散聚苯乙烯胶体的阵列，其布拉格反射可见光谱区域中的光。水凝胶随着葡萄糖浓度的变化而改变体积。这改变了晶格间距，从而改变了衍射光的波长。与我们以前的葡萄糖传感光子晶体材料相比，我们不再需要Na+螯合剂。这些光子晶体材料被设计用于糖尿病患者的葡萄糖传感接触透镜。我们描述了加快这些PCCA传感材料响应动力学的方法。快速响应动力学是通过控制水凝胶系统的弹性和水-疏水平衡来实现的。通过将丙烯酸正己酯共聚到丙烯酰胺-双丙烯酰胺水凝胶中获得更疏水的水凝胶组合物。响应速率显著增加，在90 s内完全响应于血液中发现的平均葡萄糖浓度（5 mM），在300 s内完全响应于泪液中发现的平均葡萄糖浓度（0.15 mM）。我们发现不寻常的温度依赖性动力学，这来自葡萄糖在溶液中的变旋。结果表明，α-D-葡萄糖是结合到硼酸衍生物的葡萄糖端基异构体。在任何葡萄糖测定中必须小心，以确保已经建立葡萄糖变旋平衡。我们已经证明，传感器是响应于类似于0.15 mM的葡萄糖浓度在人工泪液溶液。
We developed new photonic crystal polymerized crystalline colloidal array ( PCCA) glucose sensing materials, which operate on the basis of formation of cross-links in the hydrogel. These materials are composed of hydrogels that embed an array of similar to 100-nm-diameter monodisperse polystyrene colloids that Bragg diffract light in the visible spectral region. The hydrogels change volume as the glucose concentration varies. This changes the lattice spacing, which changes the wavelength of the diffracted light. In contrast to our previous glucose sensing photonic crystal materials, we no longer require Na+ chelating agents. These photonic crystal materials are being designed for use in glucose sensing contact lens for people with diabetes mellitus. We describe methods to speed up the response kinetics of these PCCA sensing materials. Rapid-response kinetics is achieved by controlling the elasticity and the hydrophilic-hydrophobic balance of the hydrogel system. A more hydrophobic hydrogel composition is obtained by copolymerizing n-hexylacrylate into an acrylamide-bisacrylamide hydrogel. The response rate significantly increases to where it fully responds within 90 s to the average glucose concentrations found in blood ( 5 mM) and within 300 s to the average glucose concentrations found in tear fluid (0.15 mM). We find unusual temperature-dependent kinetics, which derive from glucose mutarotation in solution. It is shown that alpha-D-glucose is the glucose anomer binding to the boronic acid derivative. Care must be taken in any glucose determination to ensure that the glucose mutarotation equilibrium has been established. We have demonstrated that the sensor is responsive to similar to 0.15 mM glucose concentrations in artificial tear fluid solution.