Isolation and characterization of human colonic bacteria able to hydrolyse chlorogenic acid
Isolation and characterization of human colonic bacteria able to hydrolyse chlorogenic acid
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DOI：
10.1046/j.1365-2672.2001.01316.x
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发表时间：
2001-06-01
影响因子：
4
通讯作者：
Faulds, CB
中科院分区：
文献类型：
--
作者：
Couteau, D;McCartney, AL;Faulds, CB
目的：共轭羟基肉桂酸，如绿原酸（咖啡酰奎宁酸），在西方饮食中被广泛食用，咖啡是最丰富的来源之一。摄取的羟基肉桂酸酯可以基本不变地到达大肠，然后可能被本地菌群产生的酯酶水解。这项研究的目的是确定在人类大肠中释放天然抗氧化剂（如羟基肉桂酸）的细菌种类。方法与结果：对35株经绿原酸培养基厌氧间歇培养的人粪菌进行肉桂酯酶活性筛选。6株分离菌株在平板筛选试验中从其乙酯中释放羟基肉桂酸阿魏酸，并通过基因型鉴定（16S rRNA测序）鉴定为大肠杆菌（3株）、乳酸双歧杆菌和干酪乳杆菌（2株）。绿原酸水解活性主要发生在细胞内。这些产肉桂酯酶的生物缺乏其他酚降解活性。结论：结果表明，某些肠道细菌，包括一些已经被认为具有潜在健康促进作用的细菌（即属于双歧杆菌属和乳杆菌属的物种），参与了人类结肠中生物活性羟基肉桂酸的释放。研究的意义和影响：游离羟基肉桂酸，包括咖啡酸、阿魏酸和对香豆酸，在体外和动物模型中均表现出抗氧化和抗癌特性。鉴于肠道菌群在人体营养和健康中起着重要作用，酚酸的一些有益作用可能归因于参与代谢的微生物菌群。
Aims: Conjugated hydroxycinnamates, such as chlorogenic acid (caffeoyl-quinic acid), are widely consumed in a Western diet, coffee being one of the richest sources. Ingested hydroxycinnamate esters can reach the large intestine essentially unaltered, and may then be hydrolysed by esterases produced by the indigenous microflora. This study is aimed at identifying bacterial species responsible for the release of natural antioxidants, such as hydroxycinnamic acids, in the human large intestine.Methods and Results: Thirty-five isolates recovered after anaerobic batch culture incubation of human faecal bacteria in a chlorogenic acid-based medium were screened for cinnamoyl esterase activity. Six isolates released the hydroxycinnamate, ferulic acid, from its ethyl ester in a plate-screening assay, and these were identified through genotypic characterization (16S rRNA sequencing) as Escherichia coli (three isolates), Bifidobacterium lactis and Lactobacillus gasseri (two strains). Chlorogenic acid hydrolysing activities were essentially intracellular. These cinnamoyl esterase-producing organisms were devoid of other phenolic-degrading activities.Conclusions: The results show that certain gut bacteria, including some already recognized as potentially health-promoting (i.e. species belonging to the genera Bifidobacterium and Lactobacillus), are involved in the release of bioactive hydroxycinnamic acids in the human colon.Significance and Impact of the Study: Free hydroxycinnamates, including caffeic, ferulic and p-coumaric acids, exhibit antioxidant and anticarcinogenic properties both in vitro and in animal models. Given that the gut flora has a major role in human nutrition and health, some of the beneficial effects of phenolic acids may be ascribed to the microflora involved in metabolism.