The possibilities and prospects of obtaining high-resolution information (below 30 Å) on biological material using the electron microscope

The possibilities and prospects of obtaining high-resolution information (below 30 Å) on biological material using the electron microscope
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使用电子显微镜获得生物材料高分辨率信息(30 Å以下)的可能性和前景

DOI:
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发表时间:
1974
期刊:
Quarterly Reviews of Biophysics (print)
影响因子:
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通讯作者:
R. Williams
R. Williams
中科院分区:
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文献类型:
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作者:
M. Beer;J. Frank;K. Hanszen;E. Kellenberger;R. Williams

文献摘要

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Commercially available electron microscopes routinely provide resolution of some 2–4 Å, as determined on the spacing of crystalline lattices of certain stable, small-molecular substances. On biological material either macromolecules or macromolecular assemblies— ‘biologically significant’ details below some 20 Å have hitherto not been observed.we consider as ‘biologically significant’ those structural details observed or contained in electronmicrographs which are consistent with, or confirmed by, other data obtained from biochemical or functional experiments or by other physical methods (optical, magnetic, electric).