Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity.
Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity.
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DOI：
10.1038/nmeth.3728
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发表时间：
2016-03
期刊：
影响因子：
48
通讯作者：
Reik W
中科院分区：
文献类型：
--
作者：
Angermueller C;Clark SJ;Lee HJ;Macaulay IC;Teng MJ;Hu TX;Krueger F;Smallwood S;Ponting CP;Voet T;Kelsey G;Stegle O;Reik W
我们报告scM&T-seq，一种用于平行单细胞全基因组甲基化组和转录组测序的方法，允许发现转录和表观遗传变异之间的关联。对61个小鼠胚胎干细胞的分析证实了DNA甲基化和转录之间的已知联系。值得注意的是，该方法揭示了异源甲基化远端调控元件与关键多能性基因转录之间的新关联。
We report scM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing, allowing discovery of associations between transcriptional and epigenetic variation. Profiling of 61 mouse embryonic stem cells confirmed known links between DNA methylation and transcription. Notably, the method reveals novel associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.