Catalase mediates acetaldehyde formation in the striatum of free-moving rats
Catalase mediates acetaldehyde formation in the striatum of free-moving rats
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DOI：
10.1016/j.neuro.2007.05.002
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发表时间：
2007-11-01
期刊：
影响因子：
3.4
通讯作者：
Ijiri, Iwao
中科院分区：
文献类型：
--
作者：
Jamal, Mostofa;Ameno, Kiyoshi;Ijiri, Iwao
使用脑微透析，我们测量了乙醇（EtOH）和乙醛（AcH）的水平在自由活动的大鼠纹状体抑制乙醇氧化途径。大鼠单独或与4-甲基吡唑（MP，82 mg/kg，一种醇脱氢酶抑制剂）和/或过氧化氢酶抑制剂叠氮化钠（AZ，10 mg/kg）或3-氨基-1，2，4-三唑（AT，1 g/kg）和/或氰胺（CY，50 mg/kg，一种醛脱氢酶抑制剂）联合腹腔注射EtOH（1 g/kg）。结果显示，EtOH和AcH浓度在EtOH给药后30 min达到平台，然后逐渐下降4 lt。在CY + EtOH、CY + AT/AZ + EtOH和CY + 4-MP + EtOH组中鉴定出AcH。CY + EtOH诱导的AcH峰值水平为195.2 +/- 19.4 μ M，该水平显著高于研究的其他组的值。过氧化氢酶或ADH抑制剂与CY组合显著降低脑中AcH浓度。在CY + 4-MP + EtOH组中，EtOH水平达到最大值25.9 +/- 2.3 mM，并且该水平显著高于EtOH组。在检查的任何其他组中均未观察到脑EtOH水平的显著差异。研究结果强烈支持的假设，酶过氧化氢酶直接在大鼠大脑中的乙酰胆碱形成中起着重要作用。(c)2007年爱思唯尔公司All rights reserved.
Using brain microdialysis, we measured both ethanol (EtOH) and acetaldehyde (AcH) levels in the striatum of free-moving rats following the inhibition of EtOH oxidation pathways. Rats received intraperitoneal EtOH (1 g/kg) alone or in combination with 4-methylpyrazole (MP, 82 mg/kg, an alcohol dehydrogenase inhibitor), and/or catalase inhibitor sodium azide (AZ, 10 mg/kg) or 3-amino-1,2,4-triazole (AT, I g/kg), and/or cyanamide (CY, 50 mg/kg, an aldehyde dehydrogenase inhibitor). Results revealed that both EtOH and AcH concentrations reached a plateau at 30 min after a dose of EtOH, and then gradually decreased for 4 It. AcH was identified in the CY + EtOH, CY + AT/AZ + EtOH, and CY + 4-MP + EtOH groups. The CY + EtOH-induced peak AcH level was 195.2 +/- 19.4 mu M, and this level was significantly higher than the values in other groups studied. The catalase or ADH inhibitor in combination with CY lowered considerably the AcH concentration in the brain. The EtOH level reached a maximum of 25.9 +/- 2.3 mM in the CY + 4-MP + EtOH group, and this level was markedly higher than in the EtOH group. No significant difference in brain EtOH levels was seen in any of the other groups examined. The findings strongly support the assumption that the enzyme catalase plays a significant role in AcH formation directly in the rat brain. (c) 2007 Elsevier Inc. All rights reserved.