REGULATION OF DEVELOPMENT IN MYXOCOCCUS-XANTHUS - EFFECT OF 3' DOUBLEBOND 5'-CYCLIC AMP, ADP, AND NUTRITION
REGULATION OF DEVELOPMENT IN MYXOCOCCUS-XANTHUS - EFFECT OF 3' DOUBLEBOND 5'-CYCLIC AMP, ADP, AND NUTRITION
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DOI：
10.1073/pnas.72.2.518
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
ZUSMAN, DR
中科院分区：
文献类型：
--
作者：
CAMPOS, JM;ZUSMAN, DR
建立了一种研究黄色粘球菌（Myxococcus xanthus）出菇调控的方法。发现核苷酸，腺苷3 '：5'-环一磷酸（环AMP）和腺苷二磷酸（ADP），在测定条件下极大地刺激出菇。观察到非常尖锐的浓度最佳值。即使在饥饿的条件下，这些核苷酸也大大增加了聚集位点的数量。营养成分影响子实体形态。在本试验条件下，研究了氨基酸对核苷酸刺激结实的影响。L-蛋氨酸和L-异亮氨酸（1 mM）完全阻断L-苏氨酸或D，L-二氨基庚二酸协同增强这些核苷酸存在下的结果量。这些数据表明，在M. xanthus
An assay was developed to study the regulation of fruiting in Myxococcus xanthus. The nucleotides, adenosine 3':5'-cyclic monophosphate (cyclic AMP) and adenosine diphosphate (ADP), were found to greatly stimulate fruiting under the assay conditions. Very sharp concentration optima were observed. Even under conditions of starvation, these nucleotides greatly increased the number of aggregation sites. Nutrition was found to influence fruiting body morphology. The effect of amino acids on the nucleotide stimulation of fruiting was studied under our assay conditions. L-Methionine and L-isoleucine (1 mM) completely blocked either L-threonine or D,L-diaminopimelic acid synergistically enhanced the amount of fruiting in the presence of these nucleotides. The data presented suggest the existence of differentiation-related regulatory compounds in M. xanthus.