COMPARISON OF FREE-LIVING AND PARTICLE-ASSOCIATED BACTERIAL COMMUNITIES IN THE CHESAPEAKE BAY BY STABLE LOW-MOLECULAR-WEIGHT RNA ANALYSIS
COMPARISON OF FREE-LIVING AND PARTICLE-ASSOCIATED BACTERIAL COMMUNITIES IN THE CHESAPEAKE BAY BY STABLE LOW-MOLECULAR-WEIGHT RNA ANALYSIS
复制标题
DOI：
10.1128/aem.61.3.944-952.1995
复制
发表时间：
1995-03-01
影响因子：
4.4
通讯作者：
FLETCHER, M
中科院分区：
文献类型：
--
作者：
BIDLE, KD;FLETCHER, M
采用吖啶橙子直接计数法和低分子量（LMW）RNA分析法，对切萨皮克湾河口自由生活和颗粒物相关细菌群落进行了分析和比较。1992年12月，从海湾中上部的顶部和底部沃茨采集了样本。在所有采样点，自由生活的细菌占主导地位，尽管颗粒相关细菌在颗粒负荷较大的区域增加。LMW RNA（5S rRNA和tRNA）直接从自由生活，颗粒相关，总浮游细菌群落进行了分析，通过高分辨率电泳。有明显的差异之间的迁移距离的自由生活和颗粒相关的社区从同一网站，表明这两个社区的组成不同。此外，低分子量RNA的配置文件不同的深度为所有的社区检查，大概是因为垂直混合。5S rRNA的自由生活的社区从上湾和中湾地区有很大的不同。另一方面，颗粒相关的RNA非常相似，这表明选择相似社区成员的颗粒上的一致环境条件。最后，几种分离的细菌具有在其各自提取的群落5S rRNA中未检测到的5S rRNA，表明这些分离的生物体不代表优势成员。
Free-living and particle-associated bacterial communities in the Chesapeake Bay estuary were analyzed and compared by using acridine orange direct counts and low-molecular-weight (LMW) RNA analysis. Samples were taken from top and bottom waters at upper- and mid-bay sites in December 1992. Free-living bacteria dominated the bacterial numbers at ail sampling sites, although particle-associated bacteria increased in areas with greater particle loads. LMW RNAs (5S rRNA and tRNA) obtained directly from free living, particle-associated, and total bacterioplankton communities were analyzed by high-resolution electrophoresis. There were distinct differences in the migration distances between LMW RNAs of free-living and particle-associated communities taken from the same site, indicating that the two communities differ in composition. In addition, LMW RNA profiles differed minimally with depth for all of the communities examined, presumably because of vertical mixing. 5S rRNAs of free-living communities from the upper- and mid-bay regions differed considerably. Particle-associated RNAs, on the other hand, were very similar, suggesting consistent environmental conditions on particles that select for similar community members. Lastly, several isolated bacteria had 5S rRNAs that were not detected in their respective extracted community 5S rRNAs, indicating that these isolated organisms were not representative of dominant members.