A conditioned medium (CM) factor produced by chondrocytes that promotes their own differentiation.
A conditioned medium (CM) factor produced by chondrocytes that promotes their own differentiation.
复制标题
软骨细胞产生的条件培养基 (CM) 因子，可促进自身分化。
DOI：
10.1016/0012-1606(73)90089-4
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发表时间：
1973
影响因子：
2.7
通讯作者：
S. Meier
中科院分区：
文献类型：
--
作者：
M. Solursh;S. Meier
本报告提供的证据表明，鸡胚软骨细胞培养物释放到培养基中的因子（S）本身可以作用于软骨细胞，以促进其自身的分化。条件培养基（CM）刺激硫酸化粘多糖和胶原蛋白的合成，如35 SO 4或葡萄糖-14 C掺入透明质酸酶敏感性材料的增加所示。然而，蛋白质合成，DNA合成和细胞数量不受影响。虽然该因子的身份尚不清楚，但它是不可透析的，胰蛋白酶和热敏性的。该因子显然是软骨细胞的特化产物，因为它不是由未表达的软骨细胞或分化的色素视网膜培养物产生的。CM对试验培养物作用迅速，处理2小时后对35 SO 4掺入有显著影响。此外，当用新鲜培养基替换CM时，效果相对稳定并且不逆转。结果是重要的，因为它们表明软骨细胞产生促进其自身分化的因子，如在两种不同的专门产物的合成方面所定义的。
This report presents evidence demonstrating that chick embryo chondrocyte cultures release into the culture medium a factor(s) which itself can act on chondrocytes to promote their own differentiation. Conditioned medium (CM) stimulates the synthesis of both sulfated mucopolysaccharides, as shown by increased incorporation of35SO4or glucose-14C into hyaluronidase-sensitive material, and collagen. However, protein synthesis, DNA synthesis, and cell number are not affected. While the identity of the factor is not yet known, it is nondialyzable, trypsin-and heat-sensitive. The factor is evidently a specialized product of chondrocytes, because it is not made by unexpressed chondrocytes or differentiated pigmented retina cultures. CM works rapidly on test cultures and has a significant effect on35SO4incorporation after 2 hr of treatment. In addition, the effect is relatively stable and is not reversed when CM is replaced with fresh medium. The results are significant in that they demonstrate that chondrocytes produce a factor that promotes their own differentiation, as defined in terms of the synthesis of two distinct specialized products.