THE FORMATION OF A STRUCTURE WITH THE FEATURES OF SYNOVIAL LINING BY SUBCUTANEOUS INJECTION OF AIR - AN INVIVO TISSUE-CULTURE SYSTEM
THE FORMATION OF A STRUCTURE WITH THE FEATURES OF SYNOVIAL LINING BY SUBCUTANEOUS INJECTION OF AIR - AN INVIVO TISSUE-CULTURE SYSTEM
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DOI：
10.1002/path.1711340205
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发表时间：
1981-01-01
影响因子：
7.3
通讯作者：
WILLOUGHBY, DA
中科院分区：
文献类型：
--
作者：
EDWARDS, JCW;SEDGWICK, AD;WILLOUGHBY, DA
为了确定滑膜是否是由于结缔组织的机械破坏而形成的，从而可能发生在任何部位，通过重复注射空气在大鼠和小鼠身上实现了皮下结缔组织的机械破坏。由此形成的空洞形成了具有许多滑膜特征的衬里结构，通过EM、苏木精-伊红和van Gieson染色的光学显微镜、酯酶活性和Ia抗原的免疫荧光染色来判断。一个与滑膜相似的结构早在6天前就形成了，这为在各种易用刺激下研究大量传真性滑膜组织提供了一种方便的方法。
To determine if synovial membrane formation due to mechanical disruption of connective tissue, thereby possibly occurring at any site, mechanical disruption of the subcutaneous connective tissue was achieved in rats and mice by the repeated injection of air. The resulting cavities developed a lining structure with many of the features of synovial membrane as judged by EM, light microscopy using haematoxylin and eosin and van Gieson stains, esterase activity and immunofluorescent staining for Ia antigen. A structure closely resembling synovium is formed as early as 6 days, providing a convenient method for studying large quantities of facsimile synovial tissue under a wide variety of easily administered stimuli.