HHV-6 infects human aortic and heart microvascular endothelial cells, increasing their ability to secrete proinflammatory chemokines
HHV-6 infects human aortic and heart microvascular endothelial cells, increasing their ability to secrete proinflammatory chemokines
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DOI：
10.1002/jmv.10133
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发表时间：
2002-08-01
影响因子：
12.7
通讯作者：
Di Luca, D
中科院分区：
文献类型：
--
作者：
Caruso, A;Rotola, A;Di Luca, D
内皮细胞是疱疹病毒感染的重要靶细胞。为了评价人类疱疹病毒6型（HHV-6）感染的生物学效应，本研究检测了成人心脏微血管和主动脉内皮细胞对HHV-6的体外敏感性以及病毒感染诱导的单核细胞趋化蛋白1（MCP-1）和白细胞介素8（IL-8）产生的变化。逆转录-聚合酶链反应和原位聚合酶链反应分析表明，HHV-6复制在内皮细胞病变的情况下，和病毒序列存在于20%的脐静脉和10%的主动脉和1%的微血管内皮。HHV-6感染上调MCP-1和IL-8的产生，在主动脉和微血管内皮之间观察到差异。这些发现表明，内皮细胞代表了HHV-6感染的潜在储库，并且改变的趋化因子产生模式可导致免疫活性细胞的吸引和炎症过程的发展。(C)2002 Wiley-Liss，Inc.
Endothelial cells are important targets for herpesvirus infection. To evaluate the biological effects of human herpesvirus-6 (HHV-6) infection, adult heart microvascular and aortic endothelial cells were examined for in vitro susceptibility to HHV-6 and for the alterations induced by viral infection on the production of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8). Analysis by reverse transcription-polymerase chain reaction and by in situ polymerase chain reaction showed that HHV-6 replicates in endothelium in the absence of cytopathic effects, and that viral sequences were present in 20% umbilical vein and in 10% aortic and 1% microvascular endothelium. HHV-6 infection upregulated the production of MCP-1 and IL-8, with differences observed between aortic and microvascular endothelium. These findings demonstrate that endothelial cells represent a potential reservoir for HHV-6 infection, and the altered pattern of chemokine production can lead to attraction of immunocompetent cells and to the development of inflammatory processes. (C) 2002 Wiley-Liss, Inc.