Simple sequence repeat (SSR) markers are very useful for genetic applications in plants, but SSR resource for the important tree genus Casuarina L. ex Adans. is still limited. In this study, we report a novel set of 223 SSR markers in Casuarina developed
Simple sequence repeat (SSR) markers are very useful for genetic applications in plants, but SSR resource for the important tree genus Casuarina L. ex Adans. is still limited. In this study, we report a novel set of 223 SSR markers in Casuarina developed
复制标题
简单序列重复 (SSR) 标记对于植物遗传应用非常有用，但重要树种 Casuarina L. ex Adans 的 SSR 资源。
作者：
Xiuyu Xu;Changpin Zhou;Yong Zhang;Weiqiang Zhang;Xianhua Gan;Huaxin Zhang;Yong Guo;Siming Gan
简单序列重复（SSR）标记在植物遗传应用中是非常有用的，但SSR资源在树木中占有重要地位。木麻黄属。仍然有限。在本研究中，我们从GenBank的EST资源中开发了一组新的223个木麻黄SSR标记。223个EST-SSR标记在10个非亲缘关系中存在多态性。每个位点的等位基因数（Na），观察到的杂合度（Ho）为预期值。杂合度（He）和多态信息含量（PIC）平均值分别为5.5、0.72、0.86和0.63。速率。跨种可转移性为96.9% (C. glauca Sieber ex Sprengel) ~ 97.8% (C. cunninghamiana Miquel) ~ 99.1% （C. junghuhniana Miquel）。用20个EST-SSRs的a亚群成功地分型了55个中国广泛种植的马叶松无性系。这些新开发的标记在遗传和育种方面具有很大的应用潜力。木麻黄种及相关分类群。
Simple sequence repeat (SSR) markers are very useful for genetic applications in plants, but SSR resource for the important tree.genus Casuarina L. ex Adans. is still limited. In this study, we report a novel set of 223 SSR markers in Casuarina developed.from expressed sequence tag (EST) resource of GenBank. The 223 EST-SSR markers were polymorphic among 10 unrelated.individuals of C. equisetifolia L. Johnson, with the number of alleles per locus (Na), observed heterozygosity (Ho), expected.heterozygosity (He), and polymorphic information content (PIC) averaging at 5.5, 0.72, 0.86, and 0.63, respectively. The rates of.cross-species transferability ranged from 96.9% (C. glauca Sieber ex Sprengel) through 97.8% (C. cunninghamiana Miquel) to.99.1% (C. junghuhniana Miquel). Fifty-five C. equisetifolia clones widely planted in China were successfully genotyped with a.subset of 20 EST-SSRs. These newly developed markers will have a great potential for genetic and breeding applications in.Casuarina species and related taxa.