COLONIZATION OF THE STREPTOMYCIN-TREATED MOUSE LARGE-INTESTINE BY A HUMAN FECAL ESCHERICHIA-COLI STRAIN - ROLE OF ADHESION TO MUCOSAL RECEPTORS
COLONIZATION OF THE STREPTOMYCIN-TREATED MOUSE LARGE-INTESTINE BY A HUMAN FECAL ESCHERICHIA-COLI STRAIN - ROLE OF ADHESION TO MUCOSAL RECEPTORS
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DOI：
10.1128/iai.56.5.1036-1043.1988
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发表时间：
1988-05-01
影响因子：
3.1
通讯作者：
COHEN, PS
中科院分区：
文献类型：
--
作者：
WADOLKOWSKI, EA;LAUX, DC;COHEN, PS
大肠杆菌F-18是一种正常的粪便分离株，以前被证明是链霉素处理的CD-1小鼠大肠的一种很好的定殖者，而E. coliF-18 col-的衍生物。大肠杆菌F-18不再产生E. coli F-18大肠杆菌素，是一个贫穷的小鼠殖民者。结果表明，E. coli F-18与可溶性结肠粘液蛋白的结合能力是E. coli F-18 col-，CD-1小鼠结肠粘液的主要受体是50.5千道尔顿的糖蛋白。在本研究中，一个额外的E。大肠杆菌F-18结肠粘液糖蛋白受体（66千道尔顿）和三个盲肠粘液糖蛋白受体（94、73和66千道尔顿）被鉴定。许多大肠杆菌特异性的结肠和盲肠刷状缘蛋白受体。coli F-18。此外，E. coli F-18col-与E. coli F-18，但程度要小得多。E.大肠杆菌F-18和F-18 col-被D-甘露糖和α-甲基-D-甘露糖苷，并且两种染色剂均显示特异性结合甘露糖-牛血清白蛋白糖缀合物的甘露糖部分，尽管同样是E. coliF-18 col结合程度较低。最后，E. coli F-18和F-18 col-1均具有纤毛。菌毛介导的粘附在大肠杆菌中的可能作用。大肠杆菌F-18定植的链霉素处理的小鼠大肠进行了讨论。
Escherichia coli F-18, a normal fecal isolate, was previously shown to be an excellent colonizer of the streptomycin-treated CD-1 mouse large intestine, whereas E. coli F-18col-, a derivative of E. coli F-18 that no longer makes the E. coli F-18 colicin, was shown to be a poor mouse colonizer. It was also shown that E. coli F-18 bound two to three times more soluble colonic mucus protein than did E. coli F-18col- and that a major receptor to CD-1 mouse colonic mucus was a 50.5-kilodalton glycoprotein. In the present investigation, an additional E. coli F-18 colonic mucus glycoprotein receptor (66 kilodaltons) and three cecal mucus glycoprotein receptors (94, 73, and 66 kilodaltons) were identified. Numerous colonic and cecal brush border protein receptors specific for E. coli F-18 were also identified. Furthermore, E. coli F-18col- was found to bind to the same mucus and brush border receptors as E. coli F-18, although to a far lesser extent. Adhesion of both E. coli F-18 and F-18col- was inhibited by D-mannose and .alpha.-methyl-D-mannoside, and both stains were shown to bind specificially to the mannose moiety of a mannose-bovine serum albumin glyconjugate, although again E. coli F-18col- bound to a lesser extent. Finally, both E. coli F-18 and F-18col- were shown to be piliated. The possible role of pilus mediated adhesion in E. coli F-18 colonization of the streptomycin-treated mouse large intestine is discussed.