Intracellular trafficking pathway of BK Virus in human renal proximal tubular epithelial cells.
Intracellular trafficking pathway of BK Virus in human renal proximal tubular epithelial cells.
复制标题
BK 病毒在人肾近端肾小管上皮细胞中的细胞内运输途径。
DOI：
10.1016/j.virol.2007.09.030
复制
发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Sorokin,Andrey
中科院分区：
文献类型：
--
作者：
Moriyama,Takahito;Sorokin,Andrey
BK病毒（BKV）在人肾近端小管上皮细胞（HRPTEC）中的细胞内运输是BKV肾炎的关键。然而，BKV使用的主要贩运部分仍然不明。HRPTEC与BKV和微管破坏剂的共孵育防止了BKV感染，如通过免疫荧光和用识别BKV大T抗原的抗体的蛋白质印迹分析所检测的。然而，抑制动力蛋白，细胞运动蛋白，不干扰BKV感染HRPTEC。BKV与内质网（ER）和高尔基体（GA）的标记物的共定位研究表明，BKV到达ER从6至10小时，而绕过GA或通过GA太短暂而无法检测。本研究有助于了解BKV在HRPTEC感染中的细胞内运输机制。
Intracellular trafficking of BK Virus (BKV) in human renal proximal tubular epithelial cells (HRPTEC) is critical for BKV nephritis. However, the major trafficking components utilized by BKV remain unknown. Coincubation of HRPTEC with BKV and microtubule disrupting agents prevented BKV infection as detected by immunofluorescence and western blot analysis with antibodies which recognize BKV large T antigen. However, inhibition of a dynein, cellular motor protein, did not interfere with BKV infection in HRPTEC. A colocalization study of BKV with the markers of the endoplasmic reticulum (ER) and the Golgi apparatus (GA), indicated that BKV reached the ER from 6 to 10 h, while bypassing the GA or passing through the GA too transiently to be detected. This study contributes to the understanding of mechanisms of intracellular trafficking used by BKV in the infection of HRPTEC.