Ca2+ buffering and action potential-evoked Ca2+ signaling in dendrites of pyramidal neurons
Ca2+ buffering and action potential-evoked Ca2+ signaling in dendrites of pyramidal neurons
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DOI：
10.1016/s0006-3495(96)79653-4
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发表时间：
1996-02-01
影响因子：
3.4
通讯作者：
Sakmann, B
中科院分区：
文献类型：
--
作者：
Helmchen, F;Imoto, K;Sakmann, B
利用体细胞全细胞记录仪和电荷耦合器件相机研究了荧光Ca2+指示染料Fura-2对大鼠大脑新皮层V层近端根尖树突和海马CA1锥体神经元Ca2+动态的影响。当Fura-2浓度从20 μ m增加到250 μ m时，单个动作电位引起树突内钙浓度（[Ca2+](i)）的短暂增加，而当Fura-2浓度从20 μ m增加到250 μ m时，这种增加的幅度减小并延长，外推到Fura-2浓度为零时，表明37℃时的“生理”瞬变具有大振幅（150-300 nM）和快速衰减（时间常数）
The effect of the fluorescent Ca2+ indicator dye Fura-2 on Ca2+ dynamics was studied in proximal apical dendrites of neocortical layer V and hippocampal CA1 pyramidal neurons in rat brain slices using somatic whole-cell recording and a charge-coupled device camera. A single action potential evoked a transient increase of intradendritic calcium concentration ([Ca2+](i)) that was reduced in size and prolonged when the Fura-2 concentration was increased from 20 to 250 mu M. Extrapolation to zero Fura-2 concentration suggests that ''physiological'' transients at 37 degrees C have large amplitudes (150-300 nM) and fast decays (time constant