PATHWAYS OF ROOT UPTAKE AND MEMBRANE TRANSPORT OF CD2+ IN THE ZINC/CADMIUM HYPERACCUMULATING PLANT SEDUM PLUMBIZINCICOLA
PATHWAYS OF ROOT UPTAKE AND MEMBRANE TRANSPORT OF CD2+ IN THE ZINC/CADMIUM HYPERACCUMULATING PLANT SEDUM PLUMBIZINCICOLA
复制标题
锌/镉超富集植物景天根系吸收和膜转运 cd2 的途径
DOI：
10.1002/etc.3625
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发表时间：
2017-04-01
影响因子：
4.1
通讯作者：
Luo, Yong-Ming
中科院分区：
文献类型：
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作者：
Li, Lian-Zhen;Tu, Chen;Luo, Yong-Ming
超积累植物紫萼景天根对镉的吸收和膜转运Guo et S.B. Zhou，前L.H. Wu通过应用扫描离子选择性电极技术评估各种抑制剂和离子通道阻滞剂对Cd积累的影响以及根部真实的时间净Cd 2+通量来表征。解偶联剂2，4-二硝基苯酚和P-型腺苷三磷酸酶抑制剂Na 3VO 4显著限制了S.铅藻属这些结果表明，镉是积极采取了根。Ca ~（2+）通道阻断剂La ~（3+）或Gd ~（3+）和K ~+通道阻断剂四乙铵预处理以及高浓度Ca ~（2+）和K ~+处理均能显著降低植株体内Cd含量。这些结果表明，根对Cd ~（2+）的吸收与根对Cd ~（2+）的吸收有关。在离子通道抑制剂的处理中，以及在Ca 2+和K+浓度升高的情况下，根表面的真实的-时间净Cd 2+通量的直接证据证实，Pbumbizincicola通过对Ca 2+和K+都具有渗透性的离子通道进行。此外，研究结果表明，植物螯合素和蛋白质合成介导的Cd 2+吸收的S。铅藻属这些结果加深了对Zn/Cd超积累植物S.铅藻属环境毒理化学2017;36：1038-1046。联系我们_SETAC
Uptake and membrane transport of cadmium (Cd) in roots of the hyperaccumulator Sedum plumbizincicola X.H. Guo et S.B. Zhou ex L.H. Wu was characterized by assessing the impact of various inhibitors and ion channel blockers on Cd accumulation as well as the real‐time net Cd2+ flux at the roots with application of the scanning ion‐selective electrode technique. The uncouplers 2,4‐dinitrophenol and P‐type adenosine triphosphatase inhibitor Na3VO4 significantly limited Cd2+ uptake and transport kinetics in the root of S. plumbizincicola. These findings indicate that Cd is actively taken up into the roots. The Cd content in plant was significantly decreased with pretreatments of the Ca2+ channel blocker La3+ or Gd3+ and the K+ channel blocker tetraethylammonium, as well as in the presence of higher concentration of Ca2+ and K+. These findings indicated that uptake of Cd2+ into the root of S. plumbizincicola proceeds through ion channels that are permeable to both Ca2+ and K+ as confirmed by the direct evidence of real‐time net Cd2+ fluxes at the root surface in the treatments with ion channel inhibitors, as well as in the presence of elevated concentrations of Ca2+ and K+. In addition, the results suggested a role for phytochelatin and protein synthesis in mediating Cd2+ uptake by S. plumbizincicola. These findings increase the understanding of Cd2+ uptake and membrane transport pathways in roots of the Zn/Cd hyperaccumulator S. plumbizincicola. Environ Toxicol Chem 2017;36:1038–1046. © 2016 SETAC