High FOXP3+ regulatory T-cell density in the sentinel lymph node is associated with downstream non-sentinel lymph-node metastasis in gastric cancer.
High FOXP3+ regulatory T-cell density in the sentinel lymph node is associated with downstream non-sentinel lymph-node metastasis in gastric cancer.
复制标题
DOI：
10.1038/bjc.2011.248
复制
发表时间：
2011-07-26
影响因子：
8.8
通讯作者：
Lee, H. S.
中科院分区：
文献类型：
--
作者：
Lee, H. E.;Park, D. J.;Kim, W. H.;Kim, H. H.;Lee, H. S.
我们的目的是评估胃癌患者前哨淋巴结 (SLN) 的免疫学性质，并确定它是否可以预测非 SLN 转移。前哨淋巴结样本采集自 64 名接受胃切除术并进行前哨淋巴结活检的胃癌患者。从每位患者中选取一份有代表性的 SLN 样本，并对其进行 CD8、CD57、FOXP3 和 DC-LAMP 免疫染色。对每个样品中标记物阳性细胞的数量进行计数。研究了各种免疫细胞密度与 SLN 和非 SLN 的临床病理参数或转移状态之间的关系。发现 SLN 的高 FOXP3+ Treg 密度与 SLN 或非 SLN 中转移的存在显着相关。 SLN 的 DC-LAMP+ 细胞密度在分离的肿瘤细胞水平上最高，并且随着 SLN 或非 SLN 中肿瘤转移的增加而降低。单变量和多变量逻辑回归模型显示，SLN 的高 FOXP3+ Treg 密度是非 SLN 转移的独立显着预测因子。这项研究首次表明前哨淋巴结在胃癌转移扩散中的重要作用。我们的研究结果表明Tregs可能成为调节胃癌转移的新治疗靶点。
We aimed to evaluate the immunologic nature of sentinel lymph nodes (SLNs) in gastric cancer patients and to determine whether it can predict non-SLN metastasis. Sentinel lymph node samples were collected from 64 gastric carcinoma patients who had undergone gastrectomy with SLN biopsy. One representative SLN sample was selected from each patient and was subjected to immunostaining for CD8, CD57, FOXP3, and DC-LAMP. The numbers of marker-positive cells in each sample were counted. The relationships between various immune cell densities and clinicopathologic parameters or metastasis status of SLNs and non-SLNs were sought. High FOXP3+ Treg density of the SLN was found to be significantly associated with the presence of metastasis in either SLNs or non-SLNs. DC-LAMP+ cell density of the SLN was the highest at the isolated tumours cell level, and this decreased along with an increase in tumour metastasis in either SLNs or non-SLNs. Univariate and multivariate logistic regression models revealed that high FOXP3+ Treg density of the SLN was an independently significant predictor of non-SLN metastasis. This study is the first to indicate an important role of SLNs in metastatic dissemination of gastric cancer. Our findings suggest that Tregs could be a new therapeutic target for regulating the metastasis of gastric cancer.
登录
查看更多内容
影响因子：
9.2
作者：
Shu, Suyu;Cochran, Alistair J.;Maecker, Holden T.
通讯作者：
Maecker, Holden T.
影响因子：
3.8
作者：
Mansfield AS;Heikkila PS;Vaara AT;von Smitten KA;Vakkila JM;Leidenius MH
通讯作者：
Leidenius MH
影响因子：
9
作者：
Ichikura, Takashi;Sugasawa, Hidekazu;Ono, Satoshi
通讯作者：
Ono, Satoshi
影响因子：
4.4
作者：
Kuwashima, N;Nishimura, F;Okada, H
通讯作者：
Okada, H
影响因子：
6.2
作者：
Matsuura, K;Yamaguchi, Y;Toge, T
通讯作者：
Toge, T