Quantitative characterization of prostaglandins in the uterus of early pregnant cattle.
Quantitative characterization of prostaglandins in the uterus of early pregnant cattle.
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DOI：
10.1530/rep-09-0081
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发表时间：
2009-08
期刊：
影响因子：
3.8
通讯作者：
S. Ulbrich;Katy Schulke;Anna E. Groebner;H. Reichenbach;C. Angioni;G. Geisslinger;H. Meyer
中科院分区：
文献类型：
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作者：
S. Ulbrich;Katy Schulke;Anna E. Groebner;H. Reichenbach;C. Angioni;G. Geisslinger;H. Meyer
前列腺素（PGs）是生殖过程（包括早期胚胎发育）的重要调节因子。我们分析了最相关的PG在牛子宫在不同的植入前妊娠阶段相比，非妊娠对照。此外，子宫内膜和滋养层组织进行了检查有关特定的酶和受体参与PG的产生和功能。对西门塔尔小母牛进行人工授精或仅接受精浆。在第12、15或18天，冲洗发情后子宫，通过液相色谱-串联质谱法测定PG。对子宫内膜和滋养层组织进行RNA提取和定量实时PCR分析。在检查的所有日期和时间点，6-酮PGF（1 α）（PGI（2）的稳定代谢物）的浓度占主导地位，其次是PGF（2 α）>PGE（2）>PGD（2），大约是TXB（2）（TXA（2）的稳定代谢物）。在第15天和第18天，PG从第12天的总体低水平增加，在妊娠期间增加更明显。PGF（2 α）/PGE（2）比值不受状态的影响。在第15天测量到最高PG浓度，6-酮PGF（1 α）（6.4 ng/ml），其次是PGF（2 α）（1.1 ng/ml）和PGE（2）（0.3 ng/ml）。子宫内膜PG生物合成酶的微小变化发生由于怀孕。滋养层细胞显示了对子宫PG有贡献的一般性和特异性PG激酶的高转录丰度。由于PGI（2）和PGF（2 α）受体在滋养层细胞中大量表达，子宫腔中大量的PGI（2）和PGF（2 α）表明PG对发育中的胚胎具有重要作用。着床前子宫中除PGE（2）外的大量PG可能对成功的生殖是必要的，而不是有害的。
Prostaglandins (PGs) are important regulators of reproductive processes including early embryonic development. We analyzed the most relevant PG in bovine uteri at different preimplantation pregnancy stages when compared with non-pregnant controls. Additionally, endometrium and trophoblast tissues were examined regarding specific enzymes and receptors involved in PG generation and function. Simmental heifers were artificially inseminated or received seminal plasma only. At days 12, 15, or 18, post-estrus uteri were flushed for PG determination by liquid chromatography-tandem mass spectrometry. Endometrium and trophoblast tissues were sampled for RNA extraction and quantitative real-time PCR analysis. At all days and points of time examined, the concentration of 6-keto PGF(1alpha) (stable metabolite of PGI(2)) was predominant followed by PGF(2alpha)>PGE(2)>PGD(2) approximately TXB(2) (stable metabolite of TXA(2)). At days 15 and 18, PG increased from overall low levels at day 12, with a much more pronounced increase during pregnancy. The PGF(2alpha)/PGE(2) ratio was not influenced by status. The highest PG concentration was measured at day 15 with 6-keto PGF(1alpha) (6.4 ng/ml) followed by PGF(2alpha) (1.1 ng/ml) and PGE(2) (0.3 ng/ml). Minor changes in endometrial PG biosynthesis enzymes occurred due to pregnancy. Trophoblasts revealed high transcript abundance of general and specific PG synthases contributing to uterine PG. As PGI(2) and PGF(2alpha) receptors were abundantly expressed by the trophoblast, abundant amounts of PGI(2) and PGF(2alpha) in the uterine lumen point towards an essential role of PG for the developing embryo. High amounts of PG other than PGE(2) in the preimplantation uterus may be essential rather than detrimental for successful reproduction.