A CONTINUOUS T-CELL LINE FROM A PATIENT WITH SEZARY-SYNDROME
A CONTINUOUS T-CELL LINE FROM A PATIENT WITH SEZARY-SYNDROME
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DOI：
10.1007/bf00431220
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发表时间：
1987-01-01
影响因子：
3
通讯作者：
STERRY, W
中科院分区：
文献类型：
--
作者：
KALTOFT, K;BISBALLE, S;STERRY, W
来自 Sezary 综合征患者的连续细胞系 Se-Ax 已经建立。 Se-Ax细胞系是IL-2依赖性的，需要人血清才能永久生长，并具有以下表型：CD1-、CD2+、CD3+、CD4-、CD5-、CD8-、CD20-、CD25+；它表达 T9、T10 和 HLA-DR 抗原。该细胞系显示出多种染色体畸变，如外周血中最丰富的异常克隆中所见。因此，它不太可能源自肿瘤细胞。来自同一患者的推定细胞毒性细胞系对自体永久细胞系仅具有微弱的杀伤细胞活性：该 CD8+ 细胞毒性细胞系具有 14q+ 染色体标记。患者没有表现出针对 Se-Ax 细胞系的自然杀伤细胞或活化杀伤细胞活性，这一事实可能部分解释了从批量培养中成功建立连续细胞系的原因。
A continuous cell line, Se-Ax, from a patient with Sezary syndrome has been established. The Se-Ax cell line is IL-2 dependent, requires human serum for permanent growth, and has the following phenotype: CD1-, CD2+, CD3+, CD4-, CD5-, CD8-, CD20-, CD25+; it expresses the T9, T10, and HLA-DR antigens. This cell line reveals multiple chromosome aberrations as seen in the most abundant abnormal clone in peripheral blood. Therefore, it is not unlikely that it derives from tumor cells. A putative cytotoxic cell line derived from the same patient has only weak killer-cell activity against the autologous permanent cell line: this CD8+ cytotoxic cell line has a 14q+ chromosomal marker. The fact that the patient demonstrated no natural killer-cell or activated killer-cell activity against the Se-Ax cell line may in part explain the successful establishment of the continuous cell line from bulk culture.