Comprehensive search for DNA polymerase in the hyperthermophilic archaeon, pyrococcus furiosus
Comprehensive search for DNA polymerase in the hyperthermophilic archaeon, pyrococcus furiosus
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DOI：
10.1080/15257770600686485
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发表时间：
2006-01-01
影响因子：
1.3
通讯作者：
Ishino, Y
中科院分区：
文献类型：
--
作者：
Ishino, S;Ishino, Y
DNA聚合酶的活性进行了扫描，在激烈的热球菌细胞提取物，以确定所有的DNA聚合酶在这种生物体。对含有DNA聚合活性的三个主要组分进行Western印迹分析，结果表明每个组分中的主要活性来自三种先前鉴定的DNA聚合酶。PCNA（proliferating cell nuclear antigen）是DNA聚合酶的滑动钳，不与三种DNA聚合酶中的任何一种紧密结合。还显示了每种纯化的DNA聚合酶的引物使用偏好。结合它们的生物化学性质，讨论了这三种DNA聚合酶在细胞DNA复制过程中的作用。
DNA polymerase activities were scanned in a Pyrococcus furiosus cell extract to identify all of the DNA polymerases in this organism. Three main fractions containing DNA polymerizing activity were subjected to Western blot analyses, which revealed that the main activities in each fraction were derived from three previously identified DNA polymerases. PCNA (proliferating cell nuclear antigen), the sliding clamp of DNA polymerases, did not bind tightly to any of the three DNA polymerases. A primer usage preference was also shown for each purified DNA polymerase. Considering their biochemical properties, the roles of the three DNA polymerases during DNA replication in the cells are discussed.