HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PHENYLTHIOCARBAMYL DERIVATIVES OF AMINO-ACIDS AND SIDE-CHAIN DERIVATIZED AMINO-ACIDS
HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PHENYLTHIOCARBAMYL DERIVATIVES OF AMINO-ACIDS AND SIDE-CHAIN DERIVATIZED AMINO-ACIDS
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DOI：
10.1016/s0021-9673(01)94449-8
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发表时间：
1987-03-13
期刊：
影响因子：
--
通讯作者：
SCHWARTZ, TW
中科院分区：
文献类型：
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作者：
OHARE, MMT;TORTORA, O;SCHWARTZ, TW
报道了用异硫氰酸苯酯柱前衍生化进行氨基酸分析，并在价格低廉的Superball C8色谱柱上对苯硫代氨基甲酰衍生物进行高效液相色谱表征。蛋白质水解物中常见氨基酸的苯硫代氨基甲酰衍生物可在15分钟内分离。给出了在紧密洗脱衍生物分离方面系统优化的建议。描述了侧链衍生氨基酸的表征，例如磷酸化和硫化氨基酸，以及一系列具有生物活性的氨基酸类似物。
Amino acid analysis by precolumn derivatization with phenyl isothiocyanate and subsequent characterization of the phenylthiocarbamyl derivatives by high-performance liquid chromatography on an inexpensive Supersphere C8 column is described. The phenylthiocarbamyl derivatives of common amino acids from protein hydrolysates are separated within 15 min. Suggestions for optimization of the system with respect to the separation of closely eluting derivatives are given. The characterization of side-chain-derivatized, e.g., phosphorylated and sulphated amino acids and of a series of biologically active amino acid analogues is described.