Expression of the macrophage-specific antigen F4/80 during differentiation of mouse bone marrow cells in culture.
Expression of the macrophage-specific antigen F4/80 during differentiation of mouse bone marrow cells in culture.
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DOI：
10.1084/jem.154.3.713
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发表时间：
1981-09-01
影响因子：
15.3
通讯作者：
Gordon, S
中科院分区：
文献类型：
--
作者：
Hirsch, S;Austyn, J M;Gordon, S
我们已经确定了巨噬细胞(M Phi)特异性抗原(Ag)F4/80在培养分化过程中的表达。以L细胞条件培养液为生长因子来源的荧光激活细胞分选和克隆实验表明，祖细胞是培养和集落形成细胞中的集落单位，缺乏F4/80抗原，但能产生F4/80阳性的贴壁细胞集落。3d后，在大量液体BM培养中发现的非贴壁前体上首次出现Ag F4/80，一旦贴壁，m Phi就表达高水平的Ag F4/80和其他标志。此外，还观察了L细胞条件培养液和贴壁对抗原F4/80表达的影响。对F4/80和其他抗原、Mac-1和2.4G2(FCR)的克隆分析表明，所有独立克隆中的所有细胞都表达这些标记。这些研究证实，F4/80是mPhi发育更成熟阶段的标志，在体外成熟过程中，Ag表达逐渐增加。抗原表达的异质性可以归因于发育过程中的变异，而不是mPhi的独立亚群。
We have defined the expression of the macrophages (m phi)-specific antigen (Ag) F4/80 during differentiation in culture. The progenitor cells-the colony-forming unit in culture and cluster-forming cell- lacked Ag F4/80 but gave rise to colonies of F4/80-positive adherent m phi, as shown by fluorescence-activated cell sorting and clonal assays with L cell-conditioned medium as the source of growth factor. Ag F4/80 first appeared on a nonadherent precursor found in mass liquid BM cultures after 3 d. Once adherent, m phi expressed high levels of Ag F4/80 and other markers. The role of L cell-conditioned medium and of adherence on expression of Ag F4/80 was also examined. Clonal analysis of F4/80 and other Ag, Mac-1, and 2.4G2 (FcR) showed that all cells in all independent colonies come to express these markers. These studies establish that F4/80 is a marker for the more mature stages of m phi development and that Ag expression increases progressively during maturation in vitro. Heterogeneity of Ag expression can be ascribed to variation in development and not to independent subsets of the m phi.