Plasma and Platelet HLA in Normal Individuals: Quantitation by Competitive Enzyme-Linked Immunoassay
Plasma and Platelet HLA in Normal Individuals: Quantitation by Competitive Enzyme-Linked Immunoassay
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正常个体血浆和血小板 HLA：通过竞争性酶联免疫测定进行定量
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发表时间：
2005
期刊：
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通讯作者：
K. Kao
中科院分区：
文献类型：
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作者：
K. Kao
最近对血小板HIA的研究表明，>50%的血小板HLA抗原吸附在血小板表面，并且可能来源于血浆。据推测，血小板HLA可能与血浆HLA浓度成正比。为探讨血浆和血小板HIA之间的定量相关性，用纯化的HIA抗原和抗HLA单克隆抗体W 6/32建立了一种精确的竞争性酶联免疫分析法（ELISA），用于测定可溶性和细胞内HIA抗原。用于测定的标准曲线的有用范围为0.01至5.0 g/mL。批内和批间变异分别为7%和14%。61例健康成人血浆HLA浓度为0.25 ~ 4.1g·Lg/mL。平均血浆HIA浓度为1.47 ± 0.87 μ g/mL（±SD）。在相同的61人中测定的血小板HLA浓度范围为
Recent studies on platelet HIA indicate that >50% of platelet HLA antigens are adsorbed on the platelet surface and may be derived from plasma. It has been speculated that platelet HLA may be directly proportional to plasma HLA concentration. To determine the quantitative correlation between plasma and platelet HIA, a precise competitive enzyme-linked immunoassay (ELISA) for measurements of soluble and cellular HIA antigens was developed by using purified HIA antigens and W6/32 anti-HLA monoclonal antibody. The useful range of the standard curve for the assay was 0.01 to 5.0 g/mL. The intraassay and interassay variations were 7% and 14%, respectively. The plasma HLA concentrations measured in 61 healthy adults ranged from 0.25 to 4.1 g.Lg/mL. and the mean plasma HIA concentration was 1 .47 ± 0.87 g/mL ( ±SD). Platelet HLA concentrations determined in the same 61 persons ranged