Overexpression of maize transcription factor mEmBP-1 increases photosynthesis, biomass, and yield in rice
Overexpression of maize transcription factor mEmBP-1 increases photosynthesis, biomass, and yield in rice
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玉米转录因子 mEmBP-1 的过度表达可增加水稻的光合作用、生物量和产量
DOI：
10.1093/jxb/eraa248
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发表时间：
2020-08-06
影响因子：
6.9
通讯作者：
Zhu, Xin-Guang
中科院分区：
文献类型：
--
作者：
Perveen, Shahnaz;Qu, Mingnan;Zhu, Xin-Guang
确定提高光合能力的新方法是提高作物产量潜力的主要途径。在这里，我们报告了编码转录因子mEmBP-1的基因的过表达导致光合作用中许多基因的同时表达增加，包括编码Chl a，b结合蛋白的基因（Lhca和Lhcb），PSII（PsbR 3和PsbW）和PSI反应中心亚基（PsaK和PsaN），叶绿体ATP合酶亚基，电子传递反应组分（Fd 1和PC），以及Calvin-Benson-Bassham循环中的主要基因，包括编码Rubisco、甘油醛磷酸脱氢酶、果糖二磷酸醛缩酶、转酮醇酶和磷酸核酮糖激酶的基因。这些光合作用基因表达的增加导致了温室和田间叶片叶绿素色素、光合速率、生物量生长和谷物产量的增加。利用EMSA实验，我们发现mEmBP-1a蛋白可以直接结合到光合作用基因的启动子区域，这表明mEmBP-1a直接结合到光合作用基因的G-box结构域上调这些基因的表达。总之，我们的研究结果表明，mEmBP-1a是一个主要的光合作用调节，它可以用来提高水稻光合作用和产量在田间。
Identifying new options to improve photosynthetic capacity is a major approach to improve crop yield potential. Here we report that overexpression of the gene encoding the transcription factor mEmBP-1 led to simultaneously increased expression of many genes in photosynthesis, including genes encoding Chl a,b-binding proteins (Lhca and Lhcb), PSII (PsbR3 and PsbW) and PSI reaction center subunits (PsaK and PsaN), chloroplast ATP synthase subunit, electron transport reaction components (Fd1 and PC), and also major genes in the Calvin-Benson-Bassham cycle, including those encoding Rubisco, glyceraldehyde phosphate dehydrogenase, fructose bisphosphate aldolase, transketolase, and phosphoribulokinase. These increased expression of photosynthesis genes resulted in increased leaf chlorophyll pigment, photosynthetic rate, biomass growth, and grain yield both in the greenhouse and in the field. Using EMSA experiments, we showed that mEmBP-1a protein can directly bind to the promoter region of photosynthesis genes, suggesting that the direct binding of mEmBP-1a to the G-box domain of photosynthetic genes up-regulates expression of these genes. Altogether, our results show that mEmBP-1a is a major regulator of photosynthesis, which can be used to increase rice photosynthesis and yield in the field.