GhAGP31, a cotton non-classical arabinogalactan protein, is involved in response to cold stress during early seedling development
GhAGP31, a cotton non-classical arabinogalactan protein, is involved in response to cold stress during early seedling development
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GhAGP31 是一种棉花非经典阿拉伯半乳聚糖蛋白，参与幼苗早期发育过程中对冷胁迫的反应
DOI：
10.1111/j.1438-8677.2011.00518.x
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发表时间：
2012-05-01
期刊：
影响因子：
3.9
通讯作者：
Li, X. -B.
中科院分区：
文献类型：
--
作者：
Gong, S. -Y.;Huang, G. -Q.;Li, X. -B.
阿拉伯半乳聚糖蛋白（AGPs）是一类高度糖基化的富含羟脯氨酸的糖蛋白超家族，广泛参与植物的生长发育。从棉花中分离到一个编码非经典AGP蛋白的基因（包括其cDNA），命名为GhAGP31。GhAGP31蛋白具有非经典AGPs的保守特征：一个信号肽、N端富含组氨酸的结构域、中间富含脯氨酸的重复结构域和一个含半胱氨酸的PAC结构域。GFP荧光检测显示GhAGP31蛋白定位于细胞壁。GhAGP31主要在根、下胚轴和胚珠中表达，在其它组织中表达很少或几乎不表达。特别是，GhAGP31的表达在根中受到发育调控。进一步的研究表明，棉花根GhAGP31的表达量在低温胁迫下显著上调。在低温处理下，转基因拟南芥幼苗的根中，由GhAGP31启动子驱动的GUS基因的表达也显著增强。此外，GhAGP31在酵母和拟南芥中的过表达显著提高了酵母细胞的抗冻性和拟南芥幼苗的耐寒性。这些数据表明，GhAGP31蛋白可能参与了棉花根系发育早期对冷胁迫的响应。
Arabinogalactan proteins (AGPs), a superfamily of highly glycosylated hydroxyproline-rich glycoproteins, are widely implicated in plant growth and development. A gene (including its cDNA), designated GhAGP31, encoding a non-classical AGP protein was isolated from cotton (Gossypium hirsutum). The deduced GhAGP31 protein contains the conserved features of non-classical AGPs: a putative signal peptide, N-terminal histidine-rich stretch, middle repetitive proline-rich domain and a cysteine-containing PAC domain. GFP fluorescence assay demonstrated that GhAGP31 protein was localised on cell walls. GhAGP31 transcripts were mainly detected in roots, hypocotyls and ovules, but little or almost none were detected in other tissues. In particular, expression of GhAGP31 was developmentally regulated in roots. Further study demonstrated that GhAGP31 expression in cotton roots was remarkably up-regulated by cold stress. Expression of the GUS gene driven by the GhAGP31 promoter was also dramatically enhanced in roots of transgenic Arabidopsis seedlings under cold treatment. Additionally, overexpression of GhAGP31 in yeast and Arabidopsis significantly improved the freezing tolerance of yeast cells and cold tolerance of Arabidopsis seedlings. These data imply that GhAGP31 protein may be involved in the response to cold stress during early root development of cotton.