Indole-3-acetonitrile production from indole glucosinolates deters oviposition by Pieris rapae
Indole-3-acetonitrile production from indole glucosinolates deters oviposition by Pieris rapae
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DOI：
10.1104/pp.107.112185
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发表时间：
2008-03-01
期刊：
影响因子：
7.4
通讯作者：
Jander, Georg
中科院分区：
文献类型：
--
作者：
de Vos, Martin;Kriksunov, Ksenia L.;Jander, Georg
像许多十字花科食草动物一样，粉蝶利用芥子油苷的存在作为产卵和幼虫进食的信号。拟南芥芥子油苷相关突变体为研究这些化合物在体内影响稻瘟病菌产卵的作用提供了独特的资源。低吲哚芥子油苷cyp 79 B2 cyp 79 B3突变体收到的卵比野生型少，证实了先前的研究表明，吲哚芥子油苷是一个重要的产卵线索。过量表达epithiosspecifier蛋白的转基因植物，将硫代葡萄糖苷分解转化为腈的形成，对产卵的P. raidopheles雌性的吸引力较小。吲哚-3-甲基硫代葡萄糖苷分解产物的cyp 79 B2 cyp 79 B3突变体的外源应用表明，产卵增加吲哚-3-甲醇和吲哚-3-乙腈（IAN）减少。萝卜拟单胞菌幼虫通过使用肠道酶将硫代葡萄糖苷分解重定向到毒性较小的腈（包括IAN）而不是异硫氰酸酯来耐受十字花科食物。IAN在幼虫寄生剂中的存在有助于减少成虫在受幼虫侵染的植物上的产卵。因此，在十字花科植物中通过epithiosspecifier蛋白质产生腈，这使得植物对通性食草动物更敏感，可能是减少P. raismus和其他十字花科昆虫产卵的反适应机制。
Like many crucifer-specialist herbivores, Pieris rapae uses the presence of glucosinolates as a signal for oviposition and larval feeding. Arabidopsis thaliana glucosinolate-related mutants provide a unique resource for studying the in vivo role of these compounds in affecting P. rapae oviposition. Low indole glucosinolate cyp79B2 cyp79B3 mutants received fewer eggs than wild type, confirming prior research showing that indole glucosinolates are an important oviposition cue. Transgenic plants overexpressing epithiospecifier protein, which shifts glucosinolate breakdown toward nitrile formation, are less attractive to ovipositing P. rapae females. Exogenous application of indol-3-ylmethylglucosinolate breakdown products to cyp79B2 cyp79B3 mutants showed that oviposition was increased by indole-3-carbinol and decreased by indole-3-acetonitrile (IAN). P. rapae larvae tolerate a cruciferous diet by using a gut enzyme to redirect glucosinolate breakdown toward less toxic nitriles, including IAN, rather than isothiocyanates. The presence of IAN in larval regurgitant contributes to reduced oviposition by adult females on larvae-infested plants. Therefore, production of nitriles via epithiospecifier protein in cruciferous plants, which makes the plants more sensitive to generalist herbivores, may be a counter-adaptive mechanism for reducing oviposition by P. rapae and perhaps other crucifer-specialist insects.