Glucocorticoid regulation of mouse mammary tumor virus sequences in transgenic mice.
Glucocorticoid regulation of mouse mammary tumor virus sequences in transgenic mice.
复制标题
转基因小鼠中糖皮质激素对小鼠乳腺肿瘤病毒序列的调节。
DOI：
10.1073/pnas.82.17.5880
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Solter,D
中科院分区：
文献类型：
--
作者：
Ross,SR;Solter,D
我们已经引入了一个嵌合质粒，pLTR 2 TK，含有小鼠乳腺肿瘤病毒（MTV）的长末端重复序列（LTR）连接到单纯疱疹病毒1型胸苷激酶基因到小鼠生殖系通过显微注射。在一个小鼠品系中，胸苷激酶基因在杂合雌性的泌乳乳腺中适当表达;在这些小鼠的卵巢中也表达。在该品系的杂合雄性中，以及在来自另一种显微注射的雄性中，这些新的生殖系基因的转录发生在睾丸中，并且被糖皮质激素特异性地刺激。因此，MTV LTR可能含有允许其在泌乳乳腺和睾丸组织中表达的元件。
We have introduced a chimeric plasmid, pLTR2TK, containing the mouse mammary tumor virus (MTV) long terminal repeat (LTR) linked to the herpes simplex virus type 1 thymidine kinase gene into the mouse germ line by microinjection. In one mouse line, the thymidine kinase gene is appropriately expressed in the lactating mammary glands of heterozygous females; expression also occurs in the ovaries of these mice. In heterozygous males of this line, and in a male derived from another microinjection, transcription of these new germ line genes occurs in the testes and is specifically stimulated by glucocorticoid hormones. Thus, the MTV LTR may contain elements that allow its expression both in lactating mammary glands and in testicular tissue.