Population deviation of piggery-associated methicillin-resistant Staphylococcus aureus based on mec-associated direct repeat unit analysis.
Population deviation of piggery-associated methicillin-resistant Staphylococcus aureus based on mec-associated direct repeat unit analysis.
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DOI：
10.1016/j.meegid.2013.03.030
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发表时间：
2013-06
期刊：
影响因子：
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通讯作者：
M. Wan;T. Lauderdale;N. Kobayashi;N. Urushibara;C. Chou
中科院分区：
文献类型：
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作者：
M. Wan;T. Lauderdale;N. Kobayashi;N. Urushibara;C. Chou
猪相关耐甲氧西林金黄色葡萄球菌（MRSA）是一种潜在的人畜共患病原体。本研究构建了台湾不同地区MRSA ST9葡萄球菌染色体盒型mec （SCCmec）的种群结构和动态。从台湾西部（96株，其中5株临床分离株）、台湾中东部（22株）和澎湖岛（22株）3个种群中收集了140株MRSA（猪场135株，临床5株），并通过mec相关直接重复单元（dru）进行了鉴定。共鉴定出28种药物类型（其中24种为新型）和15种药物克隆复合物（CCs）。占优势的新型dt12w型（48.6%）在所有种群中广泛存在，可能具有较强的种群间传播能力。最小跨越网络表明，至少鉴定出两种祖先药物类型（dt11a和dt12w），并且可以区分不同群体之间的遗传。通过贝叶斯天际线图估计的无性系种群动态的时间分布表明，MRSA ST9在台湾是一个稳定的种群，具有悠久的进化历史。研究结果表明，与猪相关的MRSA ST9和人类临床MRSA ST9之间存在一些药物类型共享，这表明SCCmec存在跨物种水平传播。
Piggery-associated methicillin-resistant Staphylococcus aureus (MRSA) is a potential zoonotic pathogen. We constructed the population structure and dynamics of staphylococcal chromosome cassette mec (SCCmec) in MRSA ST9 isolates from different geographical areas of Taiwan. A total of 140 MRSA (135 piggery and 5 human clinical) isolates from three populations located in western Taiwan (n=96 including the 5 clinical isolates), central eastern Taiwan (n=22), and Penghu Island (n=22) were collected and characterized by analysis of the mec-associated direct repeat unit (dru). Twenty-eight dru types (with 24 novel) and 15 dru-Clonal Complexes (CCs) were identified. The predominant novel dt12w type (48.6%) was widespread in all populations and may have a superior ability to transmit among populations. The minimum spanning network showed that at least two ancestral dru types (dt11a and dt12w) were identified, and the genetics between different populations could be differentiated. Temporal distributions of clone population dynamics estimated through the Bayesian skyline plot indicated a stable population with a long evolutionary history for MRSA ST9 in Taiwan. Findings indicating that some dru types are shared between piggery-associated and human-clinical MRSA ST9 suggest the occurrence of cross-species horizontal transmission of SCCmec.