Rat pancreatic islet is formed by unification of multiple endocrine cell clusters
Rat pancreatic islet is formed by unification of multiple endocrine cell clusters
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DOI：
10.1002/dvdy.21359
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发表时间：
2007-12-01
影响因子：
2.5
通讯作者：
Yamashina, Shohei
中科院分区：
文献类型：
--
作者：
Hara, Akemi;Kadoya, Yuichi;Yamashina, Shohei
用连续切片显微图像三维重建大鼠胰腺胰岛的器官发生。在胚胎日(E) 12，一个主要由胰高血糖素表达细胞组成的内分泌簇在几个区域与胰腺内胚层保持联系。在E15-E17上，花团通过新形成的芽融合而扩大。虽然胰岛素表达细胞的比例增加，但它们位于细胞簇的外围。在出生当天，表达胰岛素的细胞团扩大并融合在胰岛内形成几个核心。表达胰高血糖素的细胞团扩大形成覆盖核心的薄地幔。在胰岛器官形成过程中，外分泌管系统的增殖活性较高。此外，内分泌细胞簇始终与导管上皮保持接触。我们得出结论，胰岛是由起源于导管系统不同区域的多个内分泌簇的统一产生的。讨论了幔核的形成机制。科学通报，2009(6):351 - 358。(c) 2007 Wiley-Liss, Inc。
The organogenesis of islets in rat pancreas was studied by three-dimensional reconstructions from serial section micrographs. On embryonic day (E) 12, an endocrine cluster consisting mainly of glucagon-expressing cells maintained connection with the pancreatic endoderm at several regions. On E15-E17, the cluster enlarged by fusion of newly formed buds. Although the proportion of insulin-expressing cells increased, they were located in the periphery of the cluster. On the day of birth, insulin-expressing cell clusters enlarged and fused to form several cores within the islet. The glucagon-expressing cell mass expanded to form a thin mantle covering the cores. During islet organogenesis, proliferation activity was high in the exocrine duct system. Moreover, the endocrine cell clusters maintained contact with the duct epithelium throughout. We conclude that the pancreatic islet is generated by the unification of multiple endocrine clusters originated from separate regions of the duct system. The mechanism of mantle-core formation is discussed. Developmental Dynamics 236:3451-3458, 2007. (c) 2007 Wiley-Liss, Inc.