Changes in mRNA expression of MMP-2 in the Mullerian duct of chicken embryo
Changes in mRNA expression of MMP-2 in the Mullerian duct of chicken embryo
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DOI：
10.1016/j.ygcen.2004.08.003
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发表时间：
2004-11-01
影响因子：
2.7
通讯作者：
Shimada, K
中科院分区：
文献类型：
--
作者：
Ha, YJ;Tsukada, A;Shimada, K
虽然卵巢和输卵管的不对称发育是鸟类的一个独特特征，但不对称发育的机制仍不清楚。最近，细胞外基质的降解已被认为是与哺乳动物苗勒管退化相关的重要因素。本研究旨在探讨金属蛋白酶-2（MMP-2）在鸡胚右苗勒管退化过程中的作用。在培养的第15天观察苗勒管的形态学变化，并在培养的第12、15和18天观察MMP-2的mRNA表达。形态学观察显示右苗勒管基底膜消失，并发生退行性改变。RT-PCR分析显示，右苗勒管MMP-2 mRNA的表达在孵育的第15和18天增加，与消退的时间一致。在右侧苗勒管中，孵育第4天，己烯雌酚处理可以防止退化，并且与对照组相比，观察到MMP-2表达同时减少。这些结果表明，MMP-2可能参与了鸡雌性胚胎右苗勒管的退化。(C)2004年爱思唯尔公司All rights reserved.
Although asymmetric development of the ovary and the oviduct is a unique characteristic in birds, the mechanism of asymmetric development still remains unclear. Recently, degradation of extracellular matrix has been suggested as an important factor related to the regression of the Mullerian duct in mammals. The present study was conducted to examine a possible role of metalloproteinase-2 (MMP-2) in the regression of the right Mullerian duct in the developing chicken embryo. Morphological changes in the Mullerian ducts were studied on day 15 of incubation and mRNA expresseion of MMP-2 was studied on days 12, 15, and 18 of incubation. Morphological observation demonstrated the disappearance of basement membrane in the right Mullerian duct which undergoes the regression. RT-PCR analysis showed that MMP-2 mRNA expression of the right Mullerian duct increased on days 15 and 18 of incubation coincidently with the time of regression. In the right Mullerian duct, regression was prevented by diethylstilbestrol treatment on day 4 of incubation and a coincident decrease in MMP-2 expression was observed when compared to the control group. These results suggest that MMP-2 may be involved in the regression of the right Mullerian duct in the female embryos of the chicken. (C) 2004 Elsevier Inc. All rights reserved.