Formalin-Induced c-fos Expression in the Brain of Infant Rats
Formalin-Induced c-fos Expression in the Brain of Infant Rats
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DOI：
10.1016/j.jpain.2010.09.005
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发表时间：
2011-02-01
期刊：
影响因子：
4
通讯作者：
Barr, Gordon A.
中科院分区：
文献类型：
--
作者：
Barr, Gordon A.
在胎儿、婴儿和成年大鼠中，损伤诱导了明确的行为反应，并诱导了脊髓背角的c-fos表达。关于婴儿大脑如何处理有害刺激的信息还比较有限。我们在这里描述了福尔马林诱导损伤后胎儿和婴儿大鼠大脑中Fos蛋白的外观。选择区域进行分析，特别关注成人中表达c-fos的脑位点。胎儿大脑中未发现Fos阳性细胞；注射福尔马林后，新生儿在检查的任何结构中均未显示Fos表达增加。在3日龄和14日龄时，福尔马林诱导的腹外侧髓质Fos染色显著增加。相比之下，仅在14日龄时，丘脑室旁核和内侧背核、下丘脑室旁核和中脑导水管周围灰质的Fos蛋白水平升高。我们假设这种发展模式不仅与痛觉的成熟有关，而且与婴儿对疼痛的自主和防御反应的发展有关。观点：由于婴儿对疼痛的处理方式不同于成人，对这些差异的了解有助于儿科临床实践。使用Fos表达作为大鼠神经活动的标记，我们发现大脑激活模式在出生时是不成熟的，但在14日龄时就已经形成了。(C) 2011年由美国疼痛学会出版
In the fetal, infant, and adult rat, injury induces a well-defined behavioral response and induces c-fos expression in the spinal cord dorsal horn. There is more limited information about the processing of noxious stimulation in the infant brain. We describe here the appearance of the Fos protein in the brain of fetal and infant rats following formalin-induced injury. Regions were chosen for analysis with a special focus on brain loci that express c-fos in the adult. No Fos positive cells were found in the brains of fetuses; newborns did not show increased Fos expression after formalin injection in any structure examined. At 3 and 14 days of age, there was a significant increase in Fos staining induced by formalin in the ventral lateral medulla. In contrast, paraventricular and medial dorsal nuclei of the thalamus, the paraventricular nucleus of the hypothalamus, and periaqueductal gray of the midbrain showed increased levels of Fos protein only at 14 days of age. We hypothesize that this developmental pattern is related not only to the maturation of pain perception but also to development of autonomic and defensive reactions to pain in the infant.Perspective: Because the infant processes pain differently than the adult, knowledge of those differences informs pediatric clinical practice. Using Fos expression as a marker of neural activity in the rat, we show that the pattern of brain activation is immature at birth but is in place by 14 days of age. (C) 2011 by the American Pain Society