Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal
Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal
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DOI：
10.1128/jcm.41.6.2744-2747.2003
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发表时间：
2003-06-01
影响因子：
9.4
通讯作者：
Antunes, F
中科院分区：
文献类型：
--
作者：
Alves, M;Xiao, LH;Antunes, F
通过PCR和DNA测序分析60 kDa糖蛋白基因，对人类免疫缺陷病毒感染患者、牛和野生反刍动物的微小隐孢子虫和人隐孢子虫分离株进行了鉴定。共鉴定出7个等位基因，其中3个对应于C. hominis和四个对应于C.小的一个新的等位基因被发现（IId），和一个（IIb）只在葡萄牙被发现。牛和野生反刍动物的分离物聚集在两个等位基因中。与此相反，人类分离株聚集在7个等位基因，表现出广泛的等位基因多样性。
Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C. parvum. One new allele was found (IId), and one (IIb) had only been found in Portugal. Isolates from cattle and wild ruminants clustered in two alleles. In contrast, human isolates clustered in seven alleles, showing extensive allelic diversity.