Physical and biological properties of a novel anti-adhesion material made of thermally cross-linked gelatin film: Investigation of the usefulness as anti-adhesion material
Physical and biological properties of a novel anti-adhesion material made of thermally cross-linked gelatin film: Investigation of the usefulness as anti-adhesion material
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DOI：
10.1002/jbm.b.33880
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发表时间：
2018-02-01
影响因子：
3.4
通讯作者：
Hagiwara, Akeo
中科院分区：
文献类型：
--
作者：
Horii, Tsunehito;Tsujimoto, Hiroyuki;Hagiwara, Akeo
为了创造更有用、更有效、更安全的防粘连材料，我们开发了一种热交联明胶薄膜。在这项研究中，我们检查了膜的物理性能，如物理强度和粘附性，以揭示处理性能和生物学性能，如抗粘附效果，对细胞增殖的影响，以及细胞毒性，以揭示抗粘附机制，特别是与传统的透明质酸和羧甲基纤维素膜（传统膜）相比。干、湿拉伸试验和剪切应力试验表明，明胶膜的最大拉伸应力和断裂应变明显高于常规膜。在使用盲肠粘连的大鼠模型的研究中，明胶膜的抗粘连效果显著优于常规膜的抗粘连效果上级。在细胞增殖试验中，明胶膜上的成纤维细胞数量在每个时间点增加，而在常规膜上未观察到细胞增殖。此外，在使用菌落试验和活/死试验的细胞毒性试验中，明胶膜的浸提液没有细胞毒性，而常规膜的浸提液具有相当大的细胞毒性。这些结果表明，由于明胶膜的更好的物理强度和延展性，明胶膜提供比常规膜更好的处理。此外，明胶膜具有比常规膜显著更大的抗粘附效果，而没有任何细胞毒性。因此，明胶膜作为防粘附材料是非常有利的。(c)2017年威利期刊公司J Biomed Mater Res Part B：Appl Biomater，106 B：689-696，2018。
To create more useful, effective and safer anti-adhesion materials, we developed a thermally cross-linked gelatin film. In this study, we examined the physical properties of the film such as the physical strength and the adhesiveness to reveal the handling properties and biological properties, such as the anti-adhesion effect, the influence on cell proliferation, and the cytotoxicity to reveal the anti-adhesion mechanism, especially in comparison with the conventional hyaluronic acid and carboxymethylcellulose film (the conventional film). A tensile test under dry and wet conditions and shearing stress test showed that the gelatin film has significant higher maximum tensile stress and fracture strain than the conventional film. In the study using a rat model of cecum adhesion, the anti-adhesion effect of the gelatin film was significantly superior to that of the conventional film. In the cell proliferation test, the number of fibroblast cells on the gelatin film increased at each time point, while no cell proliferation was observed on the conventional film. Furthermore, in the cytotoxicity test using a colony assay and Live/Dead assay, the extract of the gelatin film had no cytotoxicity, while the extract of the conventional film had cytotoxicity considerably. These results suggest that the gelatin film provides better handling than the conventional film, due to better physical strength and ductility of the film. In addition, the gelatin film has a significantly greater anti-adhesion effect than the conventional film without any cytotoxicity. Therefore, the gelatin film is quite favorable as an anti-adhesion material. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 689-696, 2018.