BIOCHEMICAL-CHARACTERIZATION AND SEX DIMORPHISM OF MUSCARINIC RECEPTORS IN RAT ADENOHYPOPHYSIS
BIOCHEMICAL-CHARACTERIZATION AND SEX DIMORPHISM OF MUSCARINIC RECEPTORS IN RAT ADENOHYPOPHYSIS
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DOI：
10.1159/000123176
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发表时间：
1981-01-01
影响因子：
4.1
通讯作者：
SOKOLOVSKY, M
中科院分区：
文献类型：
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作者：
AVISSAR, S;EGOZI, Y;SOKOLOVSKY, M
采用硝酸拮抗剂n -甲基-4-哌啶基苄基、3-喹啉基苄基苯基和甲基东莨菪碱研究了大鼠腺垂体中毒蕈碱受体的配体结合特性。利用拮抗剂结合的平衡分析以及与几种激动剂的竞争实验来探讨结合位点的性质。腺垂体中拮抗剂结合的性质表明结合位点的异质性，与其他大脑区域相反，在其他大脑区域中检测到均匀的毒蕈碱受体群体。一些结合特性，如亲和常数、高亲和激动剂结合位点的数量等，清楚地表明雄性和雌性大鼠之间的差异，以及雌性大鼠在不同发情周期阶段的差异。讨论了这些受体的性质及其可能的生理作用。
The ligand binding properties of muscarinic receptors present in rat adenohypophysis were studied using the tritiated antagonists N-methyl-4-piperidyl benzilate, 3-quinuclidinyl benzilate, and methylscopolamine. Equilibrium analysis of antagonists binding as well as competition experiments with several agonists were used to probe the nature of binding sites. The nature of antagonist binding in the adenohypophysis points to heterogeneity of binding sites, in contrast to other brain regions, in which homogeneous populations of muscarinic receptors were detected. Some of the binding properties, e.g. affinity constants, population of high affinity agonist binding sites, etc., clearly indicated differences between male and female rats as well as differences in female rats at various stages of estrous cycle. The nature of these receptors and their possible physiological role are discussed.