Tcf7l2 localization of putative stem/progenitor cells in mouse conjunctiva
Tcf7l2 localization of putative stem/progenitor cells in mouse conjunctiva
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小鼠结膜中推定干细胞/祖细胞的 Tcf7l2 定位
DOI：
10.1152/ajpcell.00014.2016
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发表时间：
2016-08-01
影响因子：
5.5
通讯作者：
Lu, Rong
中科院分区：
文献类型：
--
作者：
Quan, Yadan;Zhang, Xinchun;Lu, Rong
结膜的完整性和保护是视力不可或缺的。结膜细胞的自我更新能力控制结膜的稳态和再生;然而，结膜自我更新的来源和潜在机制目前尚不清楚。在这里，我们的特点的生化表型和结膜上皮细胞在成年小鼠的增殖潜力，通过检测增殖相关的签名和进行克隆分析。此外，我们发现，转录因子7样2（T细胞特异性转录因子4），在多种类型的成人干细胞中表达的DNA结合蛋白，是高度相关的基底结膜上皮细胞的增殖签名。克隆研究表明，转录因子7样2（Tcf 712）与p63 α和增殖细胞核抗原（PCNA）在增殖集落中共表达。此外，Tcf 712在体外与结膜上皮增殖同时被积极转录。总的来说，我们认为Tcf 712可能参与维持结膜上皮干/祖细胞的干/祖细胞特性，并且穹窿是分离成年小鼠高度增殖的结膜上皮细胞的最佳部位。
Conjunctival integrity and preservation is indispensable for vision. The self-renewing capacity of conjunctival cells controls conjunctival homeostasis and regeneration; however, the source of conjunctival self-renewal and the underlying mechanism is currently unclear. Here, we characterize the biochemical phenotype and proliferative potential of conjunctival epithelial cells in adult mouse by detecting proliferation-related signatures and conducting clonal analysis. Further, we show that transcription factor 7-like 2 (T-cell-specific transcription factor 4), a DNA binding protein expressed in multiple types of adult stem cells, is highly correlated with proliferative signatures in basal conjunctival epithelia. Clonal studies demonstrated that Transcription factor 7-like 2 (Tcf712) was coexpressed with p63 alpha and proliferating cell nuclear antigen (PCNA) in propagative colonies. Furthermore, Tcf712 was actively transcribed concurrently with conjunctival epithelial proliferation in vitro. Collectively, we suggest that Tcf712 may be involved in maintenance of stem/progenitor cells properties of conjunctival epithelial stem/progenitor cells, and with the fornix as the optimal site to isolate highly proliferative conjunctival epithelial cells in adult mice.