The role of the neuropeptide Y2 receptor in liporemodeling: neuropeptide Y-mediated adipogenesis and adipose graft maintenance.
The role of the neuropeptide Y2 receptor in liporemodeling: neuropeptide Y-mediated adipogenesis and adipose graft maintenance.
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DOI：
10.1097/prs.0b013e3181954c80
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发表时间：
2009-02
影响因子：
3.6
通讯作者：
Zukowska Z
中科院分区：
文献类型：
--
作者：
Baker SB;Cohen M;Kuo L;Johnson M;Al-Attar A;Zukowska Z
神经肽Y （NPY）是一种信号分子，最近被发现通过外周作用机制刺激脂肪组织生长，该机制涉及脂肪细胞和内皮细胞上的NPY 2型受体（Y2R）。本研究旨在评估Y2R激动剂在整形外科自体脂肪移植中的应用。采用小鼠和灵长类动物模型研究NPY对脂肪组织的增殖作用。通过MRI、组织学和免疫组织化学评估小鼠和猴子皮下组织应用NPY的效果。采用超声和免疫组化方法观察NPY对人脂肪异种移植体存活及胸腺小鼠血管的影响。每组6只动物用于小鼠实验，2只动物用于灵长类动物的初步研究。在小鼠和猴子皮下应用NPY刺激脂肪组织的生长，并在三个月时增加胸腺小鼠的人类脂肪异种移植存活和血管。这些数据为NPY-Y2R相互作用在脂肪形成中的关键作用提供了体内证据，并表明Y2R是激动剂化合物的潜在靶点，可用于提高脂肪移植存活或刺激新生脂肪形成
Neuropeptide Y (NPY) is a signaling molecule that was recently found to stimulate adipose tissue growth in vitro via a peripherally acting mechanism involving NPY type 2 receptor (Y2R) found on adipocytes and endothelial cells. This study aims to evaluate the translational applications of a Y2R agonist for autologous fat grafting in plastic surgery. Murine and primate animal models were used to investigate the proliferative effects of NPY on adipose tissue. The effect of applying NPY to subcutaneous tissues in mice and monkeys was assessed by MRI, histology, and immunohistochemistry. The effect of NPY on human fat xenograft survival and vascularity in athymic mice was measured by ultrasonography and immunohistochemistry. Six animals per group were used in murine experiments, and two animals were used in the pilot primate study. NPY stimulated growth of adipose tissues when applied subcutaneously in mice and monkeys, and increased human fat xenograft survival and vascularity in athymic mice at three months. These data provide in vivo evidence for a critical role for NPY-Y2R interactions in adipogenesis, and suggest Y2R as a potential target for agonist compounds that can be used to enhance fat graft survival or stimulate de novo adipogenesis