Characterization of a set of phytochrome-interacting factor-like bHLH proteins in Oryza sativa
Characterization of a set of phytochrome-interacting factor-like bHLH proteins in Oryza sativa
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DOI：
10.1271/bbb.60643
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发表时间：
2007-05-01
影响因子：
1.6
通讯作者：
Mizuno, Takeshi
中科院分区：
文献类型：
--
作者：
Nakamura, Yuko;Kato, Takahiko;Mizuno, Takeshi
模式双子叶植物拟南芥（Arabidopsis thaliana）具有光敏色素相互作用bHLH（basic Helix-Loop-Heft）因子的特征性小亚家族，其被统称为PIL（或PIF）（光敏色素相互作用因子样）家族蛋白。在这项研究中，我们鉴定和表征了一组高度同源的成员（指定OsPIL 11到OsPIL 16）在模式单子叶植物水稻（Oryza sativa）。其中一些（OsPIL 11、OsPIL 12和OsPIL 13）显示出与假定的OsPRR 1（假反应调节因子1）时钟组分相互作用的能力，就酵母双杂交测定的结果而言。结果表明，OsPIL 13的表达受昼夜节律（clock）控制，而OsPIL 15的表达在黄化幼苗开始光照后受光负调控。水稻基因OsPIL 11 ~ OsPIL 15在A. thaliana中，所得到的转基因幼苗在早期光形态建成期间显示出异常形态，具有非常长的下胚轴。这些结果表明，OsPIL是AtPIL的功能对应物（或直系同源物），已知AtPIL在A中光感受器下游紧邻位置的红光介导的（phyA和/或phyB依赖的）信号转导途径中发挥重要作用。thaliana.
The model dicotyledon Arabidopsis thaliana has a characteristic small sub-family of phytochrome-interacting bHLH (basic Helix-Loop-Helix) factors, which are collectively designated the PIL (or PIF) (PHYTOCHROME INTERACTING FACTOR-LIKE) family proteins. In this study, we identified and characterized a set of highly homologous members (designated OsPIL11 to OsPIL16) in the model monocotyledon rice (Oryza sativa). Some of them (OsPIL11, OsPIL12, and OsPIL13) showed the ability to interact with the putative OsPRR1 (PSEUDO-RESPONSE REGULATOR 1) clock component, as far as the results of yeast two-hybrid assays were concerned. It was found that the expression of OsPIL13 is under the control of circadian rhythms (clock), while the expression of OsPIL15 is negatively regulated by light upon the onset to light exposure of etiolated seedlings. When the rice genes (OsPIL11 to OsPIL15) were over-expressed in A. thaliana, the resulting transgenic seedlings displayed anomalous morphologies with very long hypocotyls during early photomorphogenesis. These results suggest the view that the identified OsPILs are functional counterparts (or orthologs) of AtPILs, which are known to play important roles in red light-mediated (phyA and/or phyB-dependent) signal transduction pathways at immediate positions downstream of the photoreceptor in A. thaliana.