Inhibition of L-deleted foot-and-mouth disease virus replication by alpha/beta interferon involves double-stranded RNA-dependent protein kinase
Inhibition of L-deleted foot-and-mouth disease virus replication by alpha/beta interferon involves double-stranded RNA-dependent protein kinase
复制标题
α/β干扰素对L缺失型口蹄疫病毒复制的抑制涉及双链RNA依赖性蛋白激酶
DOI：
10.1128/jvi.75.12.5498-5503.2001
复制
发表时间：
2001-06-01
影响因子：
5.4
通讯作者：
Grubman, MJ
中科院分区：
文献类型：
--
作者：
Chinsangaram, J;Koster, M;Grubman, MJ
We previously demonstrated that the ability of foot-and-mouth disease virus (FMDV) to form plaques in cell culture is associated with the suppression of alpha/beta interferon (IFN-alpha/beta). In the present study, we used Escherichia coli-expressed porcine and bovine IFN-alpha or -beta individually to demonstrate that each was equally effective in inhibiting FMDV replication. The block in FMDV replication appeared to be at the level of protein translation, suggesting a role for double-stranded RNA-dependent protein kinase (PKR), In support of these findings, treatment of porcine and bovine cells with 2-aminopurine, an inhibitor of PKR, increased the yield of virus 8.8- and 11.2-fold, respectively, compared to that in untreated infected cells. In addition, results of FMDV infection in mouse embryonic fibroblast cells derived from gene knockout mice lacking the gene for RNase L-/- or PKR-/- or both indicated an important role for PKR in the inhibition of FMDV replication.
