ONLINE MULTICHANNEL RAMAN-SPECTROSCOPIC DETECTION SYSTEM FOR CAPILLARY ZONE ELECTROPHORESIS
ONLINE MULTICHANNEL RAMAN-SPECTROSCOPIC DETECTION SYSTEM FOR CAPILLARY ZONE ELECTROPHORESIS
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DOI：
10.1016/s0021-9673(01)88822-1
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发表时间：
1991-03-01
期刊：
影响因子：
--
通讯作者：
MORRIS, MD
中科院分区：
文献类型：
--
作者：
CHEN, CY;MORRIS, MD
An on-line multichannel Raman spectroscopic detection system for capillary zone electrophoresis using a charge-coupled device as the detector is described. Resonant, near-resonant and non-resonant excitation Raman spectroscopies are employed. The 400 cm-1 spectral window provides adequate information to identify resolved and unresolved compounds. The use of analyte velocity reduction to allow increased data acquisition times is described. With near-resonant enhancement, the technique is shown to allow detection of 500 attomoles of methyl orange from a 5-nl injection volume, corresponding to 1 . 10(-7) M.
