Use of c-peptide as a measure of cephalic phase insulin release in humans.
Use of c-peptide as a measure of cephalic phase insulin release in humans.
复制标题
使用 C 肽作为人体头相胰岛素释放的测量方法。
DOI：
10.1016/j.physbeh.2022.113940
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发表时间：
2022
影响因子：
2.9
通讯作者：
Lim,Juyun
中科院分区：
文献类型：
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作者：
Pullicin,AlexaJ;Newsom,SeanA;Robinson,MatthewM;Lim,Juyun
头相胰岛素释放（CPIR）是在与食物相关的感觉刺激的几分钟内分泌的胰岛素的快速脉冲。了解CPIR在人类中的潜在机制一直受到其小的观察效应大小和研究内和研究之间的高变异性的阻碍。这些限制的一个促成因素可能是使用外周测量的胰岛素作为分泌胰岛素的指标，因为大部分胰岛素在输送到外周循环之前由肝脏代谢。在这里，我们研究了使用c-肽，这是共同分泌的等摩尔量的胰岛素从胰腺β细胞，作为代理胰岛素分泌在头相期间。胰岛素和c-肽的变化进行了监测，在18名成年人在两个重复的会议后，口服刺激含蔗糖明胶刺激。我们发现，平均而言，胰岛素和c肽释放遵循类似的时间过程中的头相期间，但c肽显示出更大的效果大小。重要的是，当比较不同时间段的胰岛素和C肽浓度时，我们发现C肽的变化在2分钟（r= 0.50，p = 0.03）和4分钟（r= 0.65，p = 0.003）时间点以及考虑参与者的最高C肽浓度时（r= 0.64，p = 0.004）显著相关。与此相反，在治疗过程中测得的胰岛素变化之间没有观察到显著的相关性（r= -0.06-0.35，p> 0.05）。在此，我们详细说明了在头相期间测量的胰岛素和c肽浓度的个体变异性，并确定c肽作为一个有价值的度量胰岛素分泌的胰岛素浓度旁边时，调查CPIR。
Cephalic phase insulin release (CPIR) is a rapid pulse of insulin secreted within minutes of food-related sensory stimulation. Understanding the mechanisms underlying CPIR in humans has been hindered by its small observed effect size and high variability within and between studies. One contributing factor to these limitations may be the use of peripherally measured insulin as an indicator of secreted insulin, since a substantial portion of insulin is metabolized by the liver before delivery to peripheral circulation. Here, we investigated the use of c-peptide, which is co-secreted in equimolar amounts to insulin from pancreatic beta cells, as a proxy for insulin secretion during the cephalic phase period. Changes in insulin and c-peptide were monitored in 18 adults over two repeated sessions following oral stimulation with a sucrose-containing gelatin stimulus. We found that, on average, insulin and c-peptide release followed a similar time course over the cephalic phase period, but that c-peptide showed a greater effect size. Importantly, when insulin and c-peptide concentrations were compared across sessions, we found that changes in c-peptide were significantly correlated at the 2 min (r= 0.50,p= 0.03) and 4 min (r= 0.65,p= 0.003) time points, as well as when participants’ highest c-peptide concentrations were considered (r= 0.64,p= 0.004). In contrast, no significant correlations were observed for changes in insulin measured from the sessions (r= -0.06–0.35,p> 0.05). Herein, we detail the individual variability of insulin and c-peptide concentrations measured during the cephalic phase period, and identify c-peptide as a valuable metric for insulin secretion alongside insulin concentrations when investigating CPIR.