EXPRESSION AND SEQUENCE COMPARISON OF THE ASPERGILLUS-NIGER AND ASPERGILLUS-TUBIGENSIS GENES ENCODING POLYGALACTURONASE-II
EXPRESSION AND SEQUENCE COMPARISON OF THE ASPERGILLUS-NIGER AND ASPERGILLUS-TUBIGENSIS GENES ENCODING POLYGALACTURONASE-II
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DOI：
10.1007/bf00312738
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发表时间：
1991-01-01
期刊：
影响因子：
2.5
通讯作者：
VISSER, J
中科院分区：
文献类型：
--
作者：
BUSSINK, HJD;BUXTON, FP;VISSER, J
研究了两个最近识别的物种——黑曲霉和塔宾曲霉——的多聚半乳糖醛酸酶编码pgaII基因的结构和表达。 虽然 pgaII 基因的结构非常相似，显示出 83% 的 DNA 序列同一性和 94% 的氨基酸水平同一性，但它们存在显着差异。 NH2 末端序列表明这些 PG 被制成前蛋白原，并且 PGII 前体的分泌性前肽显示出与一些其他真菌前肽的序列同源性。 pgalII 基因的表达受到碳源的强烈调节，并且 A. tubigensis 基因在 A. niger 转化体中表达和调节。 真菌 PG 与细菌和植物来源的 PG 的低相似性与特定氨基酸的可能功能作用相关。
The structure and expression of the polygalacturonase-encoding pgaII genes of two recently recognized species, Aspergillus niger and Aspergillus tubigensis, was investigated. While the structure of the pgaII genes is very similar, showing 83% DNA sequence identity and 94% identity at the amino acid level, they have diverged significantly. The NH2-terminal sequence suggests that these PGs are made as pre pro-proteins and the secretory pro-peptide of the PGII precursors shows sequence homology with some other fungal pro-peptides. The expression of the pgaII genes is strongly regulated by the carbon source and the A. tubigensis gene is expressed and regulated in A. niger transformants. The low similarity of the fungal PGs with those of bacterial and plant origin is discussed in relation to the possible functional role of specific amino acids.