Methylation and mutation analysis of p16 gene in gastric cancer
Methylation and mutation analysis of p16 gene in gastric cancer
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DOI：
10.3748/wjg.v9.i3.423
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发表时间：
2003-03-01
影响因子：
4.3
通讯作者：
Du, P
中科院分区：
文献类型：
--
作者：
Ding, Y;Le, XP;Du, P
目的：研究胃癌组织中p16基因甲基化、纯合子缺失和突变的发生率。方法：应用甲基化敏感的限制性内切酶HpaII和甲基化不敏感的限制性内切酶MspI，采用聚合酶链式反应(PCR)技术检测p16基因外显子1和外显子2的甲基化状态。结果：20例胃癌组织中，p16基因外显子1和外显子2甲基化改变的发生率分别为25%和45%，而正常组织中未发现甲基化异常。20例胃癌组织中p16基因外显子1和外显子2纯合缺失率分别为20%和10%。未发现p16基因外显子1突变，SSCP检测发现2例(10%)外显子2出现单链异常。结论：p16基因高甲基化和异常可能在胃癌的发生发展中起关键作用。P16基因外显子2的高甲基化可能在胃粘膜的癌变过程中起作用，并可能是一种较晚的事件。
AIM: To study methylation, frequencies of homozygous deletion and mutation of p16 gene in gastric carcinoma.METHODS: The methylation pattern in exon 1 and exon 2 of p16 gene was studied with polymerase chain reaction (PCR), using methylation sensitive restriction endonuclease HpaII and methylation insensitive restriction endonuclease MspI. PCR technique was used to detect homozygous deletions of exon 1 and exon 2 of p16 gene and single strand conformation polymorphism (SSCP) technique was used to detect the mutation of the gene.RESULTS: Hypermethylation changes in exon 1 and exon 2 of p16 gene were observed in 25 % and 45 % of 20 gastric cancer tissues, respectively, while no methylation abnormality was found in normal tissues. The homozygous deletion frequency of exon 1 and exon 2 of p16 gene in 20 gastric cancer tissues was 20 % and 10 %, respectively. No mutation was found in exon 1 of p16 gene, while abnormal single strands were found in 2 (10 %) cases in exon 2 as detected by SSCP.CONCLUSION: The results suggest that hypermethylation and abnormality of p16 gene may play a key role in the progress of gastric cancer. Hypermethylation of exon 2 of p16 gene may have effects on the carcinogenesis of gastric mucosa and may be a later event.