Cloning of the chicken RNA polymerase I promoter and use for reverse genetics of influenza a viruses in avian cells
Cloning of the chicken RNA polymerase I promoter and use for reverse genetics of influenza a viruses in avian cells
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DOI：
10.1128/jvi.79.21.13811-13816.2005
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发表时间：
2005-11-01
影响因子：
5.4
通讯作者：
Naffakh, N
中科院分区：
文献类型：
--
作者：
Massin, P;Rodrigues, P;Naffakh, N
迄今为止，拯救流感病毒的反向遗传学技术是基于使用人类聚合酶I （Poll）启动子来指导八种病毒rna的合成。由于Poll启动子的物种特异性，它们只能用于来自灵长类动物的细胞。本文报道了鸡Poll启动子序列的克隆和双向PoII/PoIII质粒转染禽类细胞后重组流感病毒的产生。讨论了这一新的反向遗传系统在流感病毒研究和流感疫苗生产领域的潜在贡献。
Reverse genetics techniques to rescue influenza viruses have thus far been based on the use of a human polymerase I (Poll) promoter to direct the synthesis of the eight viral RNAs. They can only be used on cells from primate origin due to the species specificity of the Poll promoter. Here we report the cloning of the chicken Poll promoter sequence and the generation of recombinant influenza virus upon transfection of bidirectional PoII/PoIII plasmids in avian cells. Potential contributions of this new reverse genetics system in the fields of influenza virus research and influenza vaccine production are discussed.