Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina
Timed Electrodeposition of PEDOT:Nafion onto Carbon Fiber-Microelectrodes Enhances Dopamine Detection in Zebrafish Retina
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DOI：
10.1149/1945-7111/aba33d
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发表时间：
2020-01-08
影响因子：
3.9
通讯作者：
Zestos, Alexander G.
中科院分区：
文献类型：
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作者：
Cho, Whirang;Liu, Favian;Zestos, Alexander G.
碳纤维微电极（CFMEs）是检测多巴胺（DA）等神经递质的标准之一。在这项研究中，我们表明，CFMEs电沉积与聚（3，4-乙撑二氧噻吩）（PEDOT）在Nafion的存在下表现出增强的灵敏度DA检测。扫描电子显微镜（SEM）显示了聚合物涂层的光滑外表面形貌，其填充在裸露的未改性碳电极的脊和凹槽中，能量色散X射线光谱（EDX）证实了PEDOT：Nafion的掺入。PEDOT：Nafion涂层CMFE表现出统计学上增强的DA灵敏度的两倍增加相比，未改性的微电极，与涂层微电极的稳定性和完整性保持至少4小时。扫描速率测试显示与峰值DA氧化电流（5 μ M）的线性关系，表明DA的PEDOT：Nafion电极的表面的吸附控制。作为原理的证明，PEDOT：Nafion涂层电极用于检测氯化钾（KCl）诱导的斑马鱼（Danio rerio）离体视网膜组织中的DA释放，从而说明它们作为生物传感器的适用性。
Carbon fiber-microelectrodes (CFMEs) are one of the standards for the detection of neurotransmitters such as dopamine (DA). In this study, we demonstrate that CFMEs electrodeposited with poly (3,4-ethylenedioxythiophene) (PEDOT) in the presence of Nafion exhibit enhanced sensitivity for DA detection. Scanning electron microscopy (SEM) revealed the smooth outer surface morphologies of polymer coatings, which filled in the ridges and grooves of the bare unmodified carbon electrode and energy-dispersive X-ray spectroscopy (EDX) confirmed PEDOT:Nafion incorporation. PEDOT:Nafion coated CMFEs exhibited a statistically enhanced two-fold increase in DA sensitivity compared to unmodified microelectrodes, with stability and integrity of the coated microelectrodes maintained for at least 4 h. A scan rate test revealed a linear relationship with peak DA oxidative current (5 mu M), indicating adsorption control of DA to the surface of the PEDOT:Nafion electrode. As proof of principle, PEDOT:Nafion coated electrodes were used to detect potassium chloride (KCl)-induced DA release in zebrafish (Danio rerio) retinal tissue ex vivo, thus illustrating their applicability as biosensors.