RESPIRATORY SYNCYTIAL VIRUS HETEROGENEITY DURING AN EPIDEMIC - ANALYSIS BY LIMITED NUCLEOTIDE SEQUENCING (SH GENE) AND RESTRICTION MAPPING (N GENE)
RESPIRATORY SYNCYTIAL VIRUS HETEROGENEITY DURING AN EPIDEMIC - ANALYSIS BY LIMITED NUCLEOTIDE SEQUENCING (SH GENE) AND RESTRICTION MAPPING (N GENE)
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DOI：
10.1099/0022-1317-72-2-349
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发表时间：
1991-02-01
影响因子：
3.8
通讯作者：
PRINGLE, CR
中科院分区：
文献类型：
--
作者：
CANE, PA;PRINGLE, CR
采用聚合酶链反应扩增了一系列呼吸道合胞病毒（RS）小疏水（SH）蛋白的编码基因，并对其进行了克隆和测序。对1956年至1989年间分离的12株RS病毒的SH基因序列进行分析，证实了先前用血清学确定的A和B两个亚群的同质性。虽然推断出的SH蛋白氨基酸序列在亚群之间只有76%相同，但亚群内推断出的氨基酸序列差异不大；亚群内核苷酸同源性在93%到99%之间。还检测了来自单一流行季节（1989年秋冬）的42株RS病毒分离株，以确定它们的相关性。对这些分离株的SH和核衣壳蛋白基因区域进行扩增，并通过直接核苷酸测序或限制性内切图进一步分析这些区域。在同一时间和同一地区，可以区分出至少6种不同的RS病毒谱系（或亚株）。
The genes encoding the small hydrophobic (SH) proteins of a series of respiratory syncytial (RS) virus strains were amplified using the polymerase chain reaction, cloned and sequenced. Analysis of the SH gene sequences from 12 RS virus strains isolated between 1956 and 1989 confirmed the homogeneity of the two subgroups, A and B, previously defined serologically. Although there is only 76% deduced amino acid sequence identity of SH proteins between subgroups, there was little variation in deduced amino acid sequeces within the subgroups; nucleotide homologies within the subgroups ranged between 93% and 99%. Forty-two isolates of RS virus from a single epidemic season (autumn/winter 1989) were also examined to determine their relatedness. For these isolates regions of both the SH and nucleocapsid protein genes of each isolate were amplified and these regions were further analysed by direct nucleotide sequencing or restriction mapping. It was possible to discriminate at least six different lineages (or substrains) of RS virus circulating at the same time and in the same locality.