NITRIC-OXIDE PREVENTS LEUKOCYTE ADHERENCE - ROLE OF SUPEROXIDE
NITRIC-OXIDE PREVENTS LEUKOCYTE ADHERENCE - ROLE OF SUPEROXIDE
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DOI：
10.1152/ajpheart.1993.265.3.h862
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发表时间：
1993-09-01
影响因子：
--
通讯作者：
KUBES, P
中科院分区：
文献类型：
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作者：
GABOURY, J;WOODMAN, RC;KUBES, P
本研究的目的是确定NO对白细胞的抗粘附作用是否与其清除体内超氧化物的能力有关。活体显微镜被用来监测白细胞粘附和流量，以及速度和数量的滚动白细胞在25- 40-妈妈静脉。超氧化物生成系统，次黄嘌呤和黄嘌呤氧化酶（HX-XO），注入未处理的动物和预处理的动物与超氧化物歧化酶（SOD）或NO供体，SIN 1的肠系膜循环。在另一系列研究中，肠系膜制备物用血小板活化因子（PAF）或白三烯B4（LTB 4）灌流，随后施用SIN 1或SOD。HX-XO输注引起滚动和粘附白细胞数量的显著增加（完全被SOD或SIN 1抑制的反应）。SOD和SIN 1均能抑制PAF诱导的白细胞粘附，但对LTB 4诱导的白细胞粘附无明显影响。SOD和SIN 1仅在与超氧阴离子形成（HX-XO和PAF，而不是LTB 4）相关的条件下抑制白细胞粘附的观察结果强烈地表明，NO的抗粘附特性与其抑制超氧阴离子的能力有关。
The objective of this study was to determine whether the antiadhesive effects of NO for leukocytes are related to its ability to scavenge superoxide in vivo. Intravital microscopy was used to monitor leukocyte adherence and flux as well as velocity and number of rolling leukocytes in 25- to 40-mum venules. The superoxide-generating system, hypoxanthine and xanthine oxidase (HX-XO), was infused into the mesenteric circulation in untreated animals and in animals pretreated with either superoxide dismutase (SOD) or the NO donor, SIN 1. In another series of studies, the mesenteric preparation was superfused with either platelet-activating factor (PAF) or leukotriene B4 (LTB4) followed by the administration of either SIN 1 or SOD. HX-XO infusion caused a significant increase in the number of rolling and adherent leukocytes (responses that were entirely inhibited by SOD or SIN 1). SOD and SIN 1 both attenuated the PAF-induced but not the LTB4-induced leukocyte adherence. The observation that both SOD and SIN 1 inhibit leukocyte adhesion only under conditions associated with superoxide formation (HX-XO and PAF, but not LTB4) strongly suggests that the antiadhesion properties of NO are related to its ability to inactivate the superoxide anion.