Biosensor based on Prussian blue nanocubes/reduced graphene oxide nanocomposite for detection of organophosphorus pesticides.
Biosensor based on Prussian blue nanocubes/reduced graphene oxide nanocomposite for detection of organophosphorus pesticides.
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DOI：
10.1039/c2nr30976a
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发表时间：
2012-07
期刊：
影响因子：
6.7
通讯作者：
Lin Zhang;Aidong Zhang;Dan Du;Yuehe Lin
中科院分区：
文献类型：
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作者：
Lin Zhang;Aidong Zhang;Dan Du;Yuehe Lin
在聚乙烯亚胺水溶液中，通过直接混合Fe(3+)和[Fe(CN)(6)]((3)-)制备了普鲁士蓝纳米立方体/还原石墨烯氧化物(PBNCs/rGO)纳米复合材料，得到了一种新型的检测有机磷农药(OPs)的乙酰胆碱酯酶(AChE)生物传感器。用X射线光电子能谱(XPS)、透射电子显微镜(TEM)和能谱分析(EDX)对所制备的纳米复合材料进行了表征。可以清楚地观察到，纳米片上已经修饰了立方的PB纳米颗粒，几乎所有的纳米颗粒都均匀地分布在还原的GO表面。没有观察到孤立的PB纳米颗粒，这表明PB纳米立方体与还原的GO之间存在强烈的相互作用，形成了PBNCs/rGO纳米复合材料。所制得的PBNCs/rGO胆碱酯酶生物传感器的峰电位负移至220 mV。与裸电极相比，过电位降低了∼460 mV，表明PBNCs/rGO对硫代胆碱的氧化具有较高的电催化活性。该AChE生物传感器对久效磷的检测具有较快的响应速度和较高的灵敏度，线性范围为1.0~600 ng mL(-1)，检出限为0.1 ng mL(-1)。这些结果表明，PBNCs/rGO杂化纳米复合材料对硫代胆碱的氧化具有很高的电催化活性，从而实现了对有机磷农药的灵敏检测。
We demonstrate a facile procedure to efficiently prepare Prussian blue nanocubes/reduced graphene oxide (PBNCs/rGO) nanocomposite by directly mixing Fe(3+) and [Fe(CN)(6)]((3)-) in the presence of GO in polyethyleneimine aqueous solution, resulting in a novel acetylcholinesterase (AChE) biosensor for detection of organophosphorus pesticides (OPs). The obtained nanocomposite was characterized by X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and energy-dispersive X-ray (EDX) microanalysis. It was clearly observed that the nanosheet has been decorated with cubic PB nanoparticles and nearly all the nanoparticles are distributed uniformly only on the surface of the reduced GO. No isolated PB nanoparticles were observed, indicating the strong interaction between PB nanocubes and the reduced GO and the formation of PBNCs/rGO nanocomposite. The obtained PBNCs/rGO based AChE biosensor make the peak potential shift negatively to 220 mV. The over-potential decreases ∼460 mV compared to that on a bare electrode, suggesting that PBNCs/rGO has a high electrocatalytic activity towards the oxidation of thiocholine. The AChE biosensor shows rapid response and high sensitivity for detection of monocrotophos with a linear range from 1.0 to 600 ng mL(-1) and a detection limit of 0.1 ng mL(-1). These results suggest that the PBNCs/rGO hybrids nanocomposite exhibited high electrocatalytic activity towards the oxidation of thiocholine, which lead to the sensitive detection of OP pesticides.