Distribution of cardiac myosin isozymes in cardiomyopathy: immunohistochemical and gene analysis.
Distribution of cardiac myosin isozymes in cardiomyopathy: immunohistochemical and gene analysis.
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心肌病中心肌肌球蛋白同工酶的分布：免疫组织化学和基因分析。
DOI：
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发表时间：
1987
期刊：
影响因子：
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通讯作者：
S. Kimata
中科院分区：
文献类型：
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作者：
Y. Yazaki;H. Tsuchimochi;M. Kurabayashi;M. Kawana;S. Kimata
我们使用单克隆抗体通过免疫组织化学方法对心肌肌球蛋白亚型进行了表征，并证明了一种独特类型的心肌肌球蛋白重链的存在，该重链在胎儿阶段占主导地位，但在出生后发育过程中受到抑制。此外，我们发现这种类型的心肌肌球蛋白重链在扩张型心肌病患者中显着表达。结果表明，扩张型心肌病的病理过程通过抑制通常发生在肌肉成熟过程中的肌球蛋白基因转换来影响心肌分化。然而，我们在扩张型心肌病人群样本中未发现肌球蛋白基因多态性。胎儿型心肌肌球蛋白表达在扩张型心肌病中的病理生理学作用将在不久的将来通过肌球蛋白的表征来阐明。
We characterized cardiac myosin isoforms by immunohistochemical approaches using monoclonal antibodies and demonstrated the existence of a distinctive type of cardiac myosin heavy chain which predominates in the fetal stage but is depressed during postnatal development. Furthermore, we showed that this type of cardiac myosin heavy chain was markedly expressed in patients with dilated cardiomyopathy. The results suggested that the pathologic process involved in dilated cardiomyopathy affects myocardial differentiation by the inhibition of the myosin gene switching that normally occurs during muscle maturation. However, we could not find any polymorphism in myosin gene in our population sample of dilated cardiomyopathy. The pathophysiological role of fetal type cardiac myosin expression in dilated cardiomyopathy will be clarified by the characterization of the myosin near future.