Alterations in circulating levels and cardiovascular tissue content of neuropeptide Y‐like immunoreactivity during the development of deoxycorticosterone acetate‐salt hypertension in the rat
Alterations in circulating levels and cardiovascular tissue content of neuropeptide Y‐like immunoreactivity during the development of deoxycorticosterone acetate‐salt hypertension in the rat
复制标题
大鼠醋酸去氧皮质酮盐高血压发生过程中神经肽Y样免疫反应性循环水平和心血管组织含量的变化
DOI：
10.1097/00004872-199112000-00297
复制
发表时间：
1991
影响因子：
4.9
通讯作者：
N. Yamaguchi
中科院分区：
文献类型：
--
作者：
P. Moreau;J. de Champlain;N. Yamaguchi
目的探讨醋酸去氧皮质酮（DOCA）盐性高血压发生过程中血浆和组织神经肽Y样免疫反应性（NPY-li）的变化及其与血压和血浆儿茶酚胺水平的关系。方法采用DOCA盐致清醒高血压大鼠模型，分别于给药1、2、3周后测定平均动脉压（MAP）、心率、组织及血浆中NPY-li水平、主动脉血浆去甲肾上腺素和肾上腺素水平。结果DOCA-盐治疗期间，高血压大鼠血浆去甲肾上腺素和NPY-li水平均随血压升高而显著升高，MAP与血浆去甲肾上腺素和NPY-li水平显著相关。血浆NPY-li水平也仅在高血压大鼠中与去甲肾上腺素水平相关，但仅在血压正常的动物中与肾上腺素水平相关。DOCA-盐处理1-3周后，肠系膜动脉和心室中的组织NPY-li含量降低，但肾上腺中的含量与正常血压大鼠无显著差异。结论在DOCA盐高血压大鼠中，血浆NPY-li水平的增加主要来源于交感神经，因为这些水平仅与循环去甲肾上腺素水平相关，并且它们与心脏和肠系膜动脉的NPY-li含量的减少相关。因此，在该实验模型中，从交感神经释放的NPY-11的增强可能有助于血压的升高和高血压的维持。
Objective To investigate the modification of plasma and tissue neuropeptide Y-like immunoreactivity (NPY-li) concentrations, in relation to blood pressure and plasma catecholamine levels, during the development of deoxycorticosterone acetate (DOCA)-salt hypertension. Methods Mean arterial pressure (MAP), heart rate, tissue and plasma NPY-li levels, and aortic norepinephrine and epinephrine plasma levels were measured in conscious DOCA-salt hypertensive rats treated for 1, 2 and 3 weeks, and in their respective normotensive controls. Results Both norepinephrine and NPY-li plasma levels increased significantly in parallel with blood pressure during DOCA-salt treatment, so that MAP was significantly correlated with plasma norepinephrine and NPY-li levels in hypertensive rats. Plasma NPY-li levels were also correlated with norepinephrine levels only in hypertensive rats, but were correlated with epinephrine levels only in normotensive animals. Tissue NPY-li content was lower in the mesenteric artery and heart ventricles after 1–3 weeks of DOCA-salt treatment, but the content in the adrenal gland was not significantly different from that in normotensive rats. Conclusions In DOCA-salt hypertensive rats, increased plasma NPY-li levels originate primarily from the sympathetic nerves, since those levels were correlated exclusively with circulating norepinephrine levels and they were associated with a reduction in NPY-li content of the heart and mesenteric artery. It is thus possible that the enhanced release of NPY-li from sympathetic nerves could contribute to the rise in blood pressure and to the maintenance of hypertension in this experimental model.