Contribution of cell surface protein antigen c of Streptococcus mutans to platelet aggregation
Contribution of cell surface protein antigen c of Streptococcus mutans to platelet aggregation
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DOI：
10.1111/j.1399-302x.2009.00521.x
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发表时间：
2009-10-01
影响因子：
--
通讯作者：
Ooshima, T.
中科院分区：
文献类型：
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作者：
Matsumoto-Nakano, M.;Tsuji, M.;Ooshima, T.
前言：变形链球菌被认为是引起感染性心内膜炎的病原体之一。本研究的目的是研究S.方法：对6株临床分离的变形链球菌和1株同基因突变株的血小板聚集特性进行研究。用聚集仪和共聚焦显微镜分析变形链球菌，以及用抗PAc血清进行血小板聚集抑制试验。PAc表达的变异株诱导血小板聚集，而PAc缺陷的突变株和两个无PAc表达的临床分离株不诱导血小板聚集。当用高剂量抗PAc血清预处理血小板时，血小板聚集率呈剂量依赖性降低，表明PAc直接与血小板结合。变形杆菌PAc参与人血小板聚集，可能是感染性心内膜炎发病机制中的毒力因子之一。
Introduction:Streptococcus mutans is considered to be one of the pathogens that cause infective endocarditis. The purpose of the present study was to examine the properties of S. mutans with regard to platelet aggregation by focusing on its high molecular protein antigen c (PAc).Methods:The platelet aggregation properties of six clinical strains and one isogenic mutant strain of S. mutans were analysed using an aggregometer and confocal microscopy, as well as with an inhibition assay of platelet aggregation using anti-PAc serum.Results:S. mutans strains with PAc expression induced platelet aggregation, while a PAc-deficient mutant and two clinical isolates with no PAc expression did not. When platelets were pretreated with higher amounts of anti-PAc serum, the platelet aggregation rate was reduced in a dose-dependent manner, indicating that PAc binds directly to platelets.Conclusion:S. mutans PAc is involved in human platelet aggregation and may be one of the virulence factors in the pathogenesis of infective endocarditis.