Macrophage colony-stimulating factor stimulates survival and chemotactic behavior in isolated osteoclasts.
Macrophage colony-stimulating factor stimulates survival and chemotactic behavior in isolated osteoclasts.
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巨噬细胞刺激因子刺激分离的破骨细胞中的存活和趋化行为。
DOI：
10.1084/jem.178.5.1733
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发表时间：
1993-11-01
期刊：
影响因子：
--
通讯作者：
Chambers TJ
中科院分区：
文献类型：
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作者：
Fuller K;Owens JM;Jagger CJ;Wilson A;Moss R;Chambers TJ
巨噬细胞集落刺激因子（M-CSF）在破骨细胞形成中起重要作用。然而，其对成熟细胞的作用尚未完全表征。我们现在报告说，M-CSF显着刺激骨细胞的运动和扩散，破骨细胞响应梯度的M-CSF的方向，和随机细胞极化后发生的各向同性曝光。M-CSF还通过防止细胞凋亡来支持破骨细胞的存活。特别地，M-CSF抑制分离的破骨细胞的骨吸收。我们发现，这主要是通过减少挖掘数量来实现的。因此，M-CSF显示出通过减少吸收骨的细胞比例来抑制吸收的倾向。我们的数据表明，除了M-CSF在提供骨细胞诱导前体中的既定作用外，M-CSF在骨吸收中的主要作用是增强骨细胞存活、迁移和趋化性。在这些过程中，再吸收功能应该受到抑制，这似乎是适当的。我们认为，一旦破骨细胞位于再吸收位点，M-CSF就会通过调节再吸收和迁移之间的平衡来继续调节破骨细胞活性，这样不仅可以控制再吸收的数量，而且可以控制再吸收的空间模式。成骨细胞系的邻近M-CSF分泌细胞。
Macrophage colony-stimulating factor (M-CSF) is known to play an important role in osteoclast formation. However, its actions on mature cells have not been fully characterized. We now report that M-CSF dramatically stimulates osteoclastic motility and spreading; osteoclasts responded to a gradient of M-CSF with orientation, and random cell polarization occurred after isotropic exposure. M-CSF also supported the survival of osteoclasts by preventing apoptosis. Paradoxically, M-CSF inhibits bone resorption by isolated osteoclasts. We found that this was effected predominantly by reduction in the number of excavations. Thus, M-CSF showed a propensity to suppress resorption through a reduction in the proportion of cells that were resorbing bone. Our data suggest that apart from the established role of M-CSF in the provision of precursors for osteoclastic induction, a major role for M-CSF in bone resorption is to enhance osteoclastic survival, migration, and chemotaxis. It seems appropriate that during these processes resorptive functions should be suppressed. We suggest that M-CSF continues to modulate osteoclastic activity once osteoclasts are on resorptive sites, through regulation of the balance between resorption and migration, such that not only the quantity, but the spatial pattern of resorption can be controlled by adjacent M-CSF- secreting cells of osteoblastic lineage.